Cell surface characterization of Yarrowia lipolytica IMUFRJ 50682
Cell surface characterization of Yarrowia lipolytica IMUFRJ 50682
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DOI：
10.1002/yea.1405
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发表时间：
2006-09-01
期刊：
影响因子：
2.6
通讯作者：
Coutinho, J. A. P.
中科院分区：
文献类型：
--
作者：
Amaral, P. F. F.;Lehocky, M.;Coutinho, J. A. P.
在目前的工作中，一个野生型菌株的解脂耶氏酵母（IMUFRJ 50682）的表面特征进行了研究。六种不同的方法来表征细胞表面-粘附到聚苯乙烯;疏水相互作用色谱（HIC）;微生物粘附溶剂（MATS）测试; zeta电位;微生物粘附碳氢化合物（MATH）测试;和接触角测量（CAM）-被用来解释Y的细胞表面行为。解脂菌（IMUFRJ 50682）。这个Y。解脂酵母与另一株Y.解脂菌菌株（W29）。主要差异与对非极性溶剂的较高细胞粘附有关。Y.解脂酵母IMUFRJ 50682似乎在这些特定的表面特性中起重要作用，因为在链霉蛋白酶作用于其细胞壁后，该酵母疏水特性持续降低。版权所有（c）2006约翰威利父子有限公司。
In the present work, the surface characteristics of a wild-type strain of Yarrowia lipolytica (IMUFRJ50682) were investigated. Six different methods to characterize cell surfaces - adhesion to polystyrene; hydrophobic interaction chromatography (HIC); microbial adhesion to solvents (MATS) test; zeta potential; microbial adhesion to hydrocarbons (MATH) test; and contact angle measurement (CAM) - were employed to explain the cell surface behaviour of Y. lipolytica (IMUFRJ50682). This Y. lipolytica strain presents significant differences at the cell surface compared with another Y. lipolytica strain (W29) previously reported in the literature. The main difference is related to the higher cell adhesion to non-polar solvents. The proteins present on the cell wall of Y. lipolytica IMUFRJ50682 seem to play an important role in these particular surface characteristics because of the consistent reduction of this yeast hydrophobic character after the action of pronase on its cell wall. Copyright (c) 2006 John Wiley & Sons, Ltd.