Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution
Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution
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DOI：
10.1016/j.polymer.2013.09.041
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发表时间：
2014-01
期刊：
影响因子：
4.6
通讯作者：
Y. Reinwald;R. Johal;A. Ghaemmaghami;F. Rose;S. Howdle;K. Shakesheff
中科院分区：
文献类型：
--
作者：
Y. Reinwald;R. Johal;A. Ghaemmaghami;F. Rose;S. Howdle;K. Shakesheff
本研究旨在研究scCO2泡沫支架的互连性和渗透性以及结构支架特性对细胞分布的影响。利用超临界流体技术制备了37 kDa、53 kDa和109 kDa PLGA（85：15）的支架。通过MicroCT定量孔径、孔径分布和孔隙率，并使用SEM测量窗口尺寸。一种新的互连算法允许在三维空间中量化支架的互连性。为了确定多孔材料的渗透性，进行了直接灌注实验，其中应用已知的流速来测量跨支架的压差。使用达西定律计算渗透率。最大的孔径，孔隙率，互连性和渗透性，从37 kDa的PLGA制成的支架。这些支架显示出不均匀的孔结构和分布，而均匀的孔结构，最小的孔径，孔隙率，互连性和渗透性，观察从109 kDa的PLGA制成的支架。通过MicroCT和MTT染色研究3T3成纤维细胞在scCO2泡沫支架中的分布。通过荧光成像进一步观察细胞。在由109 kDa PLGA制成的支架上观察到均匀的细胞分布，并且总支架体积的平均10%被粘附在其上的细胞覆盖。
This study aims to investigate interconnectivity and permeability of scCO2-foamed scaffolds and the influence of structural scaffold properties on cell distribution. Supercritical fluid technology was utilized to fabricated scaffolds from 37 kDa, 53 kDa and 109 kDa PLGA (85:15). Pore size, pore size distribution and porosity were quantified by MicroCT, and window sizes were measured using SEM. A novel interconnectivity algorithm allowed the quantification of scaffold interconnectivity in three space dimensions. To determine the permeability of porous materials direct perfusion experiments were performed, where a known flow rate was applied to measure the pressure differential across the scaffolds. The permeability was calculated using Darcy's law. Largest pore sizes, porosities, interconnectivities and permeabilities were obtained for scaffolds fabricated from 37 kDa PLGA. These scaffolds showed a heterogeneous pore structure and distribution, whereas homogeneous pore structure, smallest pore sizes, porosities, interconnectivities and permeabilities were observed for scaffolds fabricated from 109 kDa PLGA. The distribution of 3T3 fibroblasts through scCO2-foamed scaffolds was investigated by MicroCT and MTT staining. Cells were further visualized by fluorescent imaging. Uniform cell distribution was observed on scaffolds fabricated from 109 kDa PLGA and an average of 10% of the total scaffold volume was covered with cells that had adhered onto them.