The effect of hemoglobin, hematin, and iron on neutrophil inactivation in superoxide generating systems.
The effect of hemoglobin, hematin, and iron on neutrophil inactivation in superoxide generating systems.
复制标题
血红蛋白、血红素和铁对超氧化物生成系统中中性粒细胞失活的影响。
DOI：
10.1006/abbi.1994.1118
复制
发表时间：
1994
影响因子：
3.9
通讯作者：
Piette,LH
中科院分区：
文献类型：
--
作者：
Kim,YM;Yamazaki,I;Piette,LH
用黄嘌呤氧化酶和乙醛孵育大肠杆菌，能明显抑制大肠杆菌的死亡。EDTA铁促进大肠杆菌的生长，而血红素和血红蛋白则抑制大肠杆菌的生长。另一方面，当。在佛波醇肉豆蔻酸酯乙酸酯（PMA）存在下，将大肠杆菌与人中性粒细胞一起孵育，所有这些铁物质在几微摩尔浓度下加速中性粒细胞的失活，并在这样做时保护E.大肠杆菌被中性粒细胞杀死。中性粒细胞的失活伴随着脂质过氧化作用的增加和台盼蓝测定的活力的降低。这种失活抑制清除剂，如脱氧核糖，甘露醇，或硫脲。去铁胺B和5，5-二甲基-1-吡咯啉-1-氧化物（DMPO）均抑制铁-EDTA介导的失活，但对血红素或血红蛋白介导的失活无影响。钒氧（vanadyl ion）是一种有效的芬顿试剂，其作用与EDTA铁类似。这些结果使我们得出结论，中性粒细胞失活PMA刺激过程中的OH自由基在铁-EDTA和一些其他氧化物种时，血红素或血红蛋白的存在。抗坏血酸增强了这些铁离子介导的中性粒细胞的失活。过氧化氢酶在抑制中性粒细胞失活方面非常有效。超氧化物歧化酶效果不佳，但与过氧化氢酶联合使用效果最佳。
WhenEscherichia coliwas incubated with xanthine oxidase and acetaldehyde, the killing ofE. coliwas accelerated by iron-EDTA but inhibited by hematin or hemoglobin. On the other hand, whenE. coliwas incubated with human neutrophils in the presence of phorbol myristate acetate (PMA), all of these iron species at concentrations of a few micromolar accelerated the inactivation of neutrophils and in so doing protected theE. colifrom being killed by the neutrophils. The inactivation of the neutrophils was accompanied by an increase in lipid peroxidation and by a decrease in viability measured with trypan blue. This inactivation was inhibited by scavengers such as deoxyribose, mannitol, or thiourea. Desferrioxamine B and 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) both inhibited the inactivation mediated by iron-EDTA, but had no effect on the hematin- or hemoglobin-mediated inactivation. Vanadium (vanadyl ion), an effective Fenton reagent, behaved in the same way as iron-EDTA relative to the effects of DMPO on neutrophil inactivation. These results led us to conclude that neutrophils were inactivated during PMA stimulation by OH radicals in the presence of iron-EDTA and by some other oxidizing species when hematin or Hb is present. Ascorbate enhanced the inactivation of neutrophils mediated by these iron species. Catalase was very effective in inhibiting neutrophil inactivation. Superoxide dismutase was not as effective but the combination with catalase was most effective.