Two-Way Chemical Communication between Artificial and Natural Cells.
Two-Way Chemical Communication between Artificial and Natural Cells.
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DOI：
10.1021/acscentsci.6b00330
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发表时间：
2017-02-22
影响因子：
18.2
通讯作者：
Mansy SS
中科院分区：
文献类型：
--
作者：
Lentini R;Martín NY;Forlin M;Belmonte L;Fontana J;Cornella M;Martini L;Tamburini S;Bentley WE;Jousson O;Mansy SS
能够感知并向细菌发送化学信息的人造细胞尚未被制造出来。本研究表明，能够感知和合成群体信号分子的人工细胞可以与费氏弧菌、哈维弧菌、大肠杆菌和铜绿假单胞菌进行化学交流。通过荧光、发光、RT-qPCR和RNA-seq测定活性。演示了该技术的两种潜在应用。首先，通过一种细胞图灵测试来量化人工细胞模仿自然细胞的程度。在特定的测试条件下，能够感知和响应合成和释放N-3-（氧己醇）高丝氨酸内酯的人工细胞与天然的费氏弧菌高度相似。其次，构建了能够感知V. fischeri并降解P. aeruginosa群体信号分子（N-（3-氧十二烷基）高丝氨酸内酯）的人工细胞，为未来控制自然细胞复杂网络的技术奠定基础。我们发现，能够感知和合成群体信号分子的人造细胞可以与费氏弧菌、哈维弧菌、大肠杆菌和铜绿假单胞菌进行化学交流。
Artificial cells capable of both sensing and sending chemical messages to bacteria have yet to be built. Here we show that artificial cells that are able to sense and synthesize quorum signaling molecules can chemically communicate with V. fischeri, V. harveyi, E. coli, and P. aeruginosa. Activity was assessed by fluorescence, luminescence, RT-qPCR, and RNA-seq. Two potential applications for this technology were demonstrated. First, the extent to which artificial cells could imitate natural cells was quantified by a type of cellular Turing test. Artificial cells capable of sensing and in response synthesizing and releasing N-3-(oxohexanoyl)homoserine lactone showed a high degree of likeness to natural V. fischeri under specific test conditions. Second, artificial cells that sensed V. fischeri and in response degraded a quorum signaling molecule of P. aeruginosa (N-(3-oxododecanoyl)homoserine lactone) were constructed, laying the foundation for future technologies that control complex networks of natural cells. We show that artificial cells that are able to sense and synthesize quorum signaling molecules can chemically communicate with V. fischeri, V. harveyi, E. coli, and P. aeruginosa.