THE TESTIS-SPECIFIC BETA-TUBULIN SUBUNIT IN DROSOPHILA-MELANOGASTER HAS MULTIPLE FUNCTIONS IN SPERMATOGENESIS
THE TESTIS-SPECIFIC BETA-TUBULIN SUBUNIT IN DROSOPHILA-MELANOGASTER HAS MULTIPLE FUNCTIONS IN SPERMATOGENESIS
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DOI：
10.1016/0092-8674(82)90321-x
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
RAFF, EC
中科院分区：
文献类型：
--
作者：
KEMPHUES, KJ;KAUFMAN, TC;RAFF, EC
在果蝇睾丸特异性β 2-微管蛋白的结构基因中分离出四个隐性雄性不育突变。这些突变中的每一个都编码以正常水平合成的变体β 2-微管蛋白亚基，但其随后不稳定并在睾丸内迅速降解。在这样的睾丸中，正常的α。微管蛋白也以正常水平合成，然后降解。在突变雄性中，睾丸微管蛋白池相对于野生型急剧减少。在任何隐性β 2-微管蛋白突变的纯合雄性中，在合成β 2-微管蛋白之前完成的早期有丝分裂是正常的。所有微管介导的事件后，改变的亚基的表达是有缺陷的：减数分裂，核成形和装配的轴丝都未能发生。形成果蝇精子轴丝的β 2-微管蛋白亚单位在功能上不受限制，但在精子发生中具有多种功能，包括组装单线和双线微管。
Four recessive male sterile mutations were isolated in the structural gene for the testis-specific Drosophila .beta.2-tubulin. Each of these mutations encodes a variant .beta.2-tubulin subuit synthesized at normal levels, but which is subsequently unstable and rapidly degraded within the testis. In such testes, the normal .alpha. tubulins are also synthesized at normal levels and then degraded. In mutant males the testis tubulin pool is drastically reduced relative to wild-type. In males homozygous for any of the recessive .beta.2-tubulin mutations, the early mitotic divisions, which are completed before the time of synthesis of .beta.2-tubulin, are normal. All microtubule-mediated events subsequent to the expression of the altered subunit are defective: meiosis, nuclear shaping and assembly of the axoneme all fail to occur. The .beta.2-tubulin subnit that forms the Drosophila sperm axoneme is not functionally restricted but serves multiple functions in spermatogenesis, including the assembly of both singlet and doublet tubules.