ANALYSIS OF COCAINE AND COCAETHYLENE IN BLOOD AND TISSUES BY GC-NPD AND GC-ION TRAP MASS-SPECTROMETRY
ANALYSIS OF COCAINE AND COCAETHYLENE IN BLOOD AND TISSUES BY GC-NPD AND GC-ION TRAP MASS-SPECTROMETRY
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DOI：
10.1093/jat/15.5.241
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发表时间：
1991-09-01
影响因子：
2.5
通讯作者：
COFINO, J
中科院分区：
文献类型：
--
作者：
HIME, GW;HEARN, WL;COFINO, J
可卡因是可卡因的乙基同系物，是同时摄入可卡因和乙醇的可卡因使用者体内发现的一种活跃的可卡因代谢物。为了研究可卡因和可卡因的联合发生率，设计了一种分析方法，可以同时定量两种药物在全血或组织中的含量。该方法包括气相色谱- npd定量程序和离子阱质谱确认。以可卡因的丙基同质物丙基苯甲酰茶碱为内标。方法在0.05 ~ 10.0 mg/L范围内呈线性关系，检出限为0.02 mg/L。在实验室合成了可可乙烯和丙基苯甲酰茶碱。本文介绍了完整的分析程序和合成方法，并简要介绍了法医对可卡因和可卡因都进行了定量分析以及可卡因可能在死因中起一定作用的案例。在这五个病例中，血液中的可卡因浓度在0.03至1.4毫克/升之间，可卡因浓度在0.03至0.53毫克/升之间。
Cocaethylene, the ethyl homolog of cocaine, is an active cocaine metabolite found in cocaine users who simultaneously consume cocaine and ethanol. To study the combined incidence of cocaine and cocaethylene, an analytical method was devised that would simultaneously quantify both drugs in whole blood or tissues. The method includes a quantitative procedure by GC-NPD with a confirmation by ion trap mass spectrometry. Propylbenzoylecgonine, the propyl homolog of cocaine, was used as the internal standard. The quantitative method was linear from 0.05 to 10.0 mg/L with a limit of detection of 0.02 mg/L. Cocaethylene and propylbenzoylecgonine were synthesized in the laboratory. The complete analytical procedure and the method of synthesis are presented, along with brief descriptions of medical examiner cases where both cocaine and cocaethylene were quantified and where cocaethylene may have played a part in the cause of death. In the five cases presented, the cocaine concentration ranged from 0.03 to 1.4 mg/L and cocaethylene ranged from 0.03 to 0.53 mg/L in the blood.