Oncogene-induced transformation of C3H 10T1/2 cells is enhanced by tumor promoters.
Oncogene-induced transformation of C3H 10T1/2 cells is enhanced by tumor promoters.
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肿瘤启动子可增强癌基因诱导的 C3H 10T1/2 细胞转化。
DOI：
10.1126/science.6436974
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Weinstein,IB
中科院分区：
文献类型：
--
作者：
Hsiao,WL;Gattoni-Celli,S;Weinstein,IB
肿瘤启动子12- o -tetradecanoyl- phorol -13-acetate和远杀素显著促进C3H 10T1/2小鼠成纤维细胞转染克隆的人膀胱癌c-rasHoncogene后的转化。对耐药标记物的转染研究和时间过程研究表明，这种增强不仅仅是对DNA转染过程的影响。这些发现，连同对NIH 3T3成纤维细胞的平行研究，也表明动物细胞的DNA转染能力是受体细胞系、转染标记物和生长条件的功能。我们的研究结果表明，在多阶段的癌变过程中，肿瘤启动子可能补充了活化的细胞癌基因的功能。
The tumor promoters 12-O-tetradecanoyl-phorbol-13-acetate and teleocidin markedly enhanced the transformation of C3H 10T1/2 mouse fibroblasts when these cells were transfected with the cloned human bladder cancer c-rasHoncogene. Transfection studies with the drug resistance markergptand time course studies indicate that this enhancement is not simply an effect on the process of DNA transfection. These findings, together with parallel studies with NIH 3T3 fibroblasts, also indicate that the competence of animal cells for DNA transfection is a function of the recipient cell line, the transfected marker, and the growth conditions. Our findings suggest that during multistage carcinogenesis tumor promoters may complement the function of activated cellular oncogenes.