Transcriptional profiling reveals gland-specific differential expression in the three major salivary glands of the adult mouse
Transcriptional profiling reveals gland-specific differential expression in the three major salivary glands of the adult mouse
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DOI：
10.1152/physiolgenomics.00124.2017
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发表时间：
2018-04-01
影响因子：
4.6
通讯作者：
Melvin, James E.
中科院分区：
文献类型：
--
作者：
Gao, Xin;Oei, Maria S.;Melvin, James E.
RNA-Seq用于更好地了解哺乳动物三种主要外分泌唾液腺之间生物学差异的分子本质。转录谱分析发现，成年小鼠腮腺，下颌下和舌下唾液腺表达超过14，300个蛋白质编码基因，其中近2，000个基因差异表达。差异表达基因的主成分分析显示，根据腺体类型的三个不同的集群。三个唾液腺转录组中占主导地位的是相对少数的高表达基因（6.3%），占90%以上的转录输出。在大唾液腺中表达的912个转录因子中，超过90%的转录因子在所有三个腺体中被检测到，而其中2%的转录因子在单个腺体中富集。这些独特的转录因子的表达与舌下和腮腺特异性亚群的高表达和差异表达基因相关。基因本体论分析表明，所有腺体共同的高表达基因与全球功能相关，而在单个腺体中表达的许多基因在该腺体的功能中起着重要作用。总之，三种小鼠大唾液腺的转录谱鉴定了有限数量的高表达基因、差异表达基因和代表腺特异性生物学特性的转录特征的独特转录因子。
RNA-Seq was used to better understand the molecular nature of the biological differences among the three major exocrine salivary glands in mammals. Transcriptional profiling found that the adult murine parotid, submandibular, and sublingual salivary glands express greater than 14,300 protein-coding genes, and nearly 2,000 of these genes were differentially expressed. Principle component analysis of the differentially expressed genes revealed three distinct clusters according to gland type. The three salivary gland transcriptomes were dominated by a relatively few number of highly expressed genes (6.3%) that accounted for more than 90% of transcriptional output. Of the 912 transcription factors expressed in the major salivary glands, greater than 90% of them were detected in all three glands, while expression for similar to 2% of them was enriched in an individual gland. Expression of these unique transcription factors correlated with sublingual and parotid specific subsets of both highly expressed and differentially expressed genes. Gene ontology analyses revealed that the highly expressed genes common to all glands were associated with global functions, while many of the genes expressed in a single gland play a major role in the function of that gland. In summary, transcriptional profiling of the three murine major salivary glands identified a limited number of highly expressed genes, differentially expressed genes, and unique transcription factors that represent the transcriptional signatures underlying gland-specific biological properties.