Age related changes in fluidity of rat renal brushborder membrane vesicles.
Age related changes in fluidity of rat renal brushborder membrane vesicles.
复制标题
大鼠肾刷状缘膜囊泡流动性的年龄相关变化。
DOI：
10.1016/0006-291x(87)91491-4
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Segal,S
中科院分区：
文献类型：
--
作者：
Medow,MS;Segal,S
测定了1,6二苯基-1,3,5 -己三烯在7、14、21和28日龄大鼠和成人肾刷缘膜在5°C至45°C范围内的荧光各向异性。在不同年龄组中，温度和平均荧光各向异性之间存在平行关系，流动性随年龄的增长而逐渐减少。在任何年龄组的膜中都没有明显的相变，荧光偏振“断点”的缺乏证明了这一点。在限制阻碍各向异性和先前确定的Na+-脯氨酸超调高度之间也存在线性关系。这表明导致流动性差异的肾刷缘膜的物理特性与年龄依赖性转运改变有关。
Fluorescence anisotrophy of 1,6 diphenyl-1,3,5,-hexatriene was determined in renal brushborder membranes prepared from rats 7, 14, 21 and 28 days of age, and adults, from 5°C to 45°C. There is a parallel relationship between temperature and mean fluorescence anisotrophy in the different age groups with a progressive decrease in fluidity with age. There is no phase transition apparent in membranes from any age group as evidenced by the lack of a fluorescence polarization “break point”. There is also a linear relationship between limiting hindered anisotrophy and previously determined values for the height of the Na+-proline overshoot. This suggests that the physical characteristics of the renal brushborder membrane responsible for differences in fluidity are related to age-dependent transport alterations.