Effect of interleukin-11 on cycling status and clonogenic maturation of fetal and adult hematopoietic progenitors
Effect of interleukin-11 on cycling status and clonogenic maturation of fetal and adult hematopoietic progenitors
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白细胞介素11对胎儿和成人造血祖细胞循环状态和克隆成熟的影响
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
R. Christensen
中科院分区：
文献类型：
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作者：
K. Schibler;Yu;R. Christensen
最近克隆的人细胞因子白细胞介素-11（IL-11）与IL-6具有功能相似性。我们测试了IL-11在体外的造血作用也类似于IL-6的假设。IL-11对胎儿和成人造血祖细胞的细胞周期状态的影响进行了评估，使用无血清孵育，然后氚化胸苷自杀研究。通过将IL-11作为单一药物或与亚平台或平台浓度的其他重组细胞因子一起加入含有针对相关生长因子的中和单克隆抗体的培养物中，评估其对克隆形成成熟的影响。与IL-6相似，IL-11导致胎儿集落形成单位混合（CFU-MIX）、CFU-GM和红系爆发形成单位（BFU-E）的加速循环。这种效果是添加剂的次最大，但不是平台，IL-6的浓度。然而，没有观察到IL-11对成体祖细胞的循环状态的影响。作为单一试剂，IL-11不能支持胎儿或成人祖细胞的克隆成熟。IL-11与GM-CSF对成人骨髓CFU-GM克隆成熟有协同作用，但对胎儿血CFU-GM克隆成熟无协同作用。我们的结论是，在体外造血作用的IL-11对造血祖细胞的细胞周期状态类似于IL-6。然而，与IL-6不同，IL-11作为单一试剂不能支持胎儿CFU-GM、BFU-E和CFU-MIX的克隆成熟。
A recently cloned human cytokine, interleukin-11 (IL-11), has functional similarities to IL-6. We tested the hypothesis that the hematopoietic actions of IL-11 in vitro also resemble those of IL-6. The effect of IL-11 on the cell cycle status of fetal and adult hematopoietic progenitors was assessed using serum-free incubations followed by tritiated thymidine suicide studies. Its effect on clonogenic maturation was assessed by including IL-11, either as a single agent or with subplateau or plateau concentrations of other recombinant cytokines, in cultures that contained neutralizing monoclonal antibodies directed against relevant growth factors. Similar to IL-6, IL-11 resulted in accelerated cycling of fetal colony-forming units-mixed (CFU-MIX), CFU-granulocyte macrophage (CFU-GM), and erythroid burst-forming units (BFU-E). This effect was additive to that of submaximal, but not to plateau, concentrations of IL-6. However, no effect of IL-11 was observed on cycling status of adult progenitors. As a single agent, IL-11 failed to support clonal maturation of either fetal or adult progenitors. IL-11 was additive to GM-CSF in supporting clonal maturation of CFU-GM from adult marrow but not from fetal blood. We conclude that the in vitro hematopoietic actions of IL-11 on cell cycle status of hematopoietic progenitors resemble those of IL-6. However, unlike IL-6, IL-11 as a single agent failed to support clonal maturation of fetal CFU-GM, BFU-E, and CFU-MIX.
DOI：
10.1073/pnas.87.19.7512
发表时间：
1990-10-01
影响因子：
11.1
作者：
PAUL, SR;BENNETT, F;YANG, YC
通讯作者：
YANG, YC