Bioinspired Programmable Engineering of a Color-Change Biointerface based on Dual-Stimulation Regulation
Bioinspired Programmable Engineering of a Color-Change Biointerface based on Dual-Stimulation Regulation
复制标题
基于双刺激调节的变色生物界面的仿生可编程工程
DOI：
10.1021/acsabm.0c01224
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发表时间：
2020
影响因子：
4.7
通讯作者：
Fan Xia
中科院分区：
文献类型：
--
作者：
Meihua Lin;Hao Wan;Jian Zhang;Fujian Huang;Shaoguang Li;Fan Xia
一些动物可以通过控制皮肤色素细胞或鸟嘌呤晶体来改变皮肤颜色，以应对多种刺激。受这些自然特性的启发，人们开发了一种人工变色界面；然而，对不同类型的刺激作出反应的界面仍然很少见。在此，我们设计了一种变色生物界面，通过改变DNA结构的构象来响应光和DNA的协同刺激，从而实现界面的可编程激活。我们通过将荧光染料标记的发夹DNA与光响应分子结合来构建生物界面，该分子在紫外线的第一次刺激（刺激1，S1）下被切割以暴露脚位结构域。然后，在入侵DNA探针的第二次刺激（刺激2，S2）下，通过支点介导的DNA链位移反应改变生物界面的颜色。在s2存在下，随着s1从0 s增加到600 s，生物界面的转化效率从6.8 s提高到64%。此外，在S1的刺激下，复合矩阵中不同数量的s2的有效转换效率也从5.3调整到72%，这表明在现实环境中具有高响应的潜力。
Some animals can change their skin colors in response to multiple stimuli by controlling the skin pigment cell or guanine crystals. An artificial color-change interface inspired by these natural properties has been developed; the interface responded to different types of stimuli, however, is still rare. Herein, we design a color-change biointerface that responds to the cooperative stimulation of light and DNA by changing the conformation of DNA structures to achieve programmable activation of the interface. We construct the biointerface by the combination of a fluorescent dye-labeled hairpin DNA with a photoresponsive molecule, which is cleaved by the first stimulation of UV light (stimulus 1,S1) to expose the toehold domain. Then, the biointerface gets color changed through the toehold-mediated DNA strand displacement reaction with the second stimulation of the invading DNA probe (stimulus 2,S2). Moreover, the transformation efficiency of the biointerface increased from 6.8 to 64% with an increase ofS1from 0 to 600 s in the presence ofS2. Also, after the stimulation of S1, the effective transformation efficiency was also tuned from 5.3 to 72% by different amounts ofS2in the complex matrix, indicating the potential for a high response in real-world settings.