[The interaction of berberine and human serum albumin].
[The interaction of berberine and human serum albumin].
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发表时间：
2004
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通讯作者：
Chang-chun Zhao;Weiwen Zheng;Meng-qiu Li
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作者：
Chang-chun Zhao;Weiwen Zheng;Meng-qiu Li
通过荧光和紫外-可见吸收光谱研究了小檗碱和人血清白蛋白 (HSA) 的相互作用。采用荧光猝灭法测得其结合常数K为1.168×10(5)L×mol(-1)，结合位点数n为5.26。根据福斯特的非辐射能量转移理论还获得了结合距离（R = 3.44 nm）和能量转移效率（E = 0.303）。酪氨酸残基和色氨酸残基之间的能量传递路径被小檗碱在HSA位点切断，并且还观察到弱荧光的小檗碱-HSA复合物。
The interaction of berberine and Human Serum Albumin (HSA) has been studied by fluorescence and UV-Vis absorption spectroscopy. With fluorescence quenching method, the binding constant K was found to be 1.168 x 10(5) L x mol(-1) and the number of binding site n was 5.26. The binding distance (R = 3.44 nm) and energy transfer efficiency (E = 0.303) were also obtained according to Förster's non-radiation energy transfer theory. The energy transfer path between tyrosine residual and tryptophan residual was cut by berberine in the site of HSA and weak fluorescence berberine-HSA complex was also observed.