Transient pores in vesicles
Transient pores in vesicles
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DOI：
10.1002/pi.1007
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发表时间：
2003-04-01
影响因子：
3.2
通讯作者：
Brochard-Wyart, F
中科院分区：
文献类型：
--
作者：
Karatekin, E;Sandre, O;Brochard-Wyart, F
我们提出了我们的观察瞬态孔隙在巨大的单层囊泡，置于张力下，通过光学照明。当膜张力达到一定水平时，出现瞬态孔隙。通过使用甘油和水的粘性混合物，我们减缓了存在孔隙时内部液体泄漏的速度。这使得孔隙可以达到很大的尺寸（几微米），并且至少持续几秒钟，这样它们就可以通过荧光视频显微镜观察到。线张力是从孔的闭合速度的测量中推断出来的。通过添加增加T（减少孔隙寿命）的胆固醇或减少T（增加孔隙寿命）的表面活性剂，我们展示了如何将T以及因此产生的孔隙寿命控制在近两个数量级。表面活性剂的加入对囊泡融合也有显著的影响。我们讨论了如何将我们的结果扩展到粘性较低的水溶液，这与脂质体药物递送制剂更相关。(C) 2003年化学工业学会。
We present our observations of transient pores in giant unilamellar vesicles, placed under tension, by optical illumination. When the membrane tension reached a certain level, transient pores appeared. Pore opening is driven by the membrane tension, or, and its closure by the pore's line tension, T. By use of viscous mixtures of glycerol and water, we slowed down the leak out of the inner liquid in the presence of a pore. This allowed pores to reach large sizes (a few micrometres) and last at least a few seconds so that they could be visualized by fluorescence videomicroscopy. Line tension was inferred from the measurements of the closure velocity of the pores. By addition of cholesterol, which increased T (reducing pore lifetimes), or of surfactants, which decreased T (increasing pore lifetimes), we demonstrate how T, and consequently pore lifetimes, can be controlled over nearly two orders of magnitude. Addition of surfactants also has a dramatic effect on vesicle fusion. We discuss how our results can be extended to less viscous aqueous solutions which are more relevant for liposomal drug delivery formulations. (C) 2003 Society of Chemical Industry.