Regulation of growth performance and lipid metabolism by dietary n-3 highly unsaturated fatty acids in juvenile grass carp, Ctenopharyngodon idellus
Regulation of growth performance and lipid metabolism by dietary n-3 highly unsaturated fatty acids in juvenile grass carp, Ctenopharyngodon idellus
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DOI：
10.1016/j.cbpb.2011.01.009
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发表时间：
2011-05-01
影响因子：
2.2
通讯作者：
Liu, Pin
中科院分区：
文献类型：
--
作者：
Ji, Hong;Li, Jie;Liu, Pin
为了研究n-3高不饱和脂肪酸（HUFAs）对草鱼（Ctenopharyngodon idellus）的影响，采用5种等氮等能半纯化饲料，分别添加0%（对照）、0.26%、0.52%、0.83%和1.13%的n-3高不饱和脂肪酸，进行了为期75 d的饲养试验。饲料中HUFAs含量从0%增加到0.52%，增重、特定生长率、饲料效率和蛋白质效率均呈上升趋势，随后呈下降趋势。腹腔脂肪含量和肝胰脂质水平以0.52%组最低。组织脂肪酸水平与饲料HUFAs含量有良好的相关性。肝脏脂蛋白脂肪酶（LPL）活性在0.52%水平组显著升高，苹果酸脱氢酶（MDH）活性在0-0.52%水平组保持稳定，在1.13%水平组显著降低。随着饲料中HUFAs含量的增加，超氧化物歧化酶（SOD）活性显著升高，与丙二醛（MDA）水平一致。肝脏过氧化物酶体增殖物活化受体-a (PPAR-a) mRNA表达量在饲料中添加HUFAs含量为0.83%时最高，LPL基因mRNA表达量随饲料中HUFAs含量的增加而增加，最高可达0.83%。由此可见，饲料中添加足量的HUFAs可显著促进淡水鱼草鱼的生长性能和脂质代谢。然而，过量的HUFAs强化可能会产生不利影响，这可能是由于氧化应激。(C) 2011爱思唯尔公司版权所有。
To determine the effects of n-3 highly unsaturated fatty acids (HUFAs) in grass carp (Ctenopharyngodon idellus), a 75-day feeding experiment was conducted using five isonitrogenous and isoenergetic semi-purified diets containing 0% (control), 0.26%, 0.52%, 0.83% or 1.13% n-3 HUFAs. Weight gain, specific growth rate, feed efficiency and protein efficiency increased by increasing the dietary HUFAs content from 0% to 0.52%, and declined thereafter. Intraperitoneal fat content and the hepatopancreatic lipid levels were lowest in the 0.52% group. The tissue fatty acid level was well correlated with dietary HUFAs content. Hepatic lipoprotein lipase (LPL) activity was significantly higher in the 0.52% group, while that of malate dehydrogenase (MDH) was stable in the 0-0.52% groups, and was significantly lower in the 1.13% group. Superoxide dismutase (SOD) activity increased significantly with increasing dietary HUFAs content, consistent with the level of malondialdehyde (MDA). Hepatic mRNA expression of peroxisome proliferator-activated receptor-a (PPAR-a) was greatest in the 0.83% group and that of the LPL gene increased with increasing dietary HUFAs content up to 0.83%. These results indicate that adequate dietary HUFAs supplementation significantly promotes growth performance and lipid metabolism in freshwater fish grass carp. However, excess HUFAs fortification may exert adverse effects, which might be due to oxidative stress. (C) 2011 Elsevier Inc. All rights reserved.