PARINARIC ACID AS A SENSITIVE FLUORESCENT-PROBE FOR THE DETERMINATION OF LIPID-PEROXIDATION
PARINARIC ACID AS A SENSITIVE FLUORESCENT-PROBE FOR THE DETERMINATION OF LIPID-PEROXIDATION
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DOI：
10.1016/0005-2760(87)90027-0
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发表时间：
1987-09-25
期刊：
影响因子：
--
通讯作者：
DENKAMP, JAFO
中科院分区：
文献类型：
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作者：
KUYPERS, FA;VANDENBERG, JJM;DENKAMP, JAFO
共轭多烯酰基链荧光的减少被用作脂质过氧化的灵敏测定。脂肪酸顺式-反式-反式-顺-9,11,13,15-十八碳四烯酸（顺式石蜡酸）以游离脂肪酸的形式引入脂质体膜，或者通过使用来自牛肝脏的PC特异性转移蛋白，以1-棕榈酰-2-顺式-石蜡酰-sn-甘油-3-磷酸胆碱的形式引入脂质体膜。通过荧光强度的降低监测的过氧化过程与其他过氧化测定系统进行了比较。讨论了新测定系统的应用。
The decrease in fluorescence of conjugated polyenic acyl chains is used as a sensitive assay for lipid peroxidation. The fatty acid cis-trans-trans-cis-9,11,13,15-octadecatetraenoic acid (cis-parinaric acid) is introduced into liposomal membranes as free fatty acid or, by using the PC specific transfer protein from bovine liver, as 1-palmitoyl-2-cis-parinaroyl-sn-glycero-3-phosphocholine. The peroxidation process as monitored by the decrease in fluorescence intensity is compared with other peroxidation assay systems. Applications of the new assay system are discussed.