Specific expression of Gsta4 in mouse cochlear melanocytes: a novel role for hearing and melanocyte differentiation
Specific expression of Gsta4 in mouse cochlear melanocytes: a novel role for hearing and melanocyte differentiation
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DOI：
10.1111/j.1755-148x.2008.00513.x
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发表时间：
2009-02
影响因子：
4.3
通讯作者：
Shigeyuki Uehara;Y. Izumi;Y. Kubo;Chi-Chiu Wang;Katsuhiko Mineta;K. Ikeo;T. Gojobori;M. Tachibana;T. Kikuchi;Toshimitsu Kobayashi;S. Shibahara;C. Taya;H. Yonekawa;T. Shiroishi;Hiroaki Yamamoto
中科院分区：
文献类型：
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作者：
Shigeyuki Uehara;Y. Izumi;Y. Kubo;Chi-Chiu Wang;Katsuhiko Mineta;K. Ikeo;T. Gojobori;M. Tachibana;T. Kikuchi;Toshimitsu Kobayashi;S. Shibahara;C. Taya;H. Yonekawa;T. Shiroishi;Hiroaki Yamamoto
哺乳动物的色素细胞产生黑色素作为主要色素。黑素细胞是哺乳动物色素细胞的两种类型之一，在发育过程中从神经嵴分化并迁移到各种器官。黑素细胞不仅存在于皮肤中，也存在于耳蜗等对听力至关重要的部位。Mitfmi - bw是小鼠小眼相关转录因子（Mitf）基因座的已知隐性等位基因之一，对色素细胞的发育至关重要。纯合子Mitfmi - bw/Mitfmi - bw小鼠具有完全白色的皮毛和黑色的眼睛，由于缺乏黑色素细胞而失聪。通过比较野生型和Mitfmi - bw/Mitfmi - bw小鼠耳蜗的基因表达谱，我们现在证明了谷胱甘肽S -转移酶α 4 （Gsta4）在血管纹中的特异性表达。Gsta4编码一种参与许多组织解毒过程的胞质谷胱甘肽S -转移酶（GSTs）。该基因在血管纹中间细胞中特异性表达，提示耳蜗黑素细胞具有新的功能。此外，在哺乳动物色素细胞中，Gsta4的表达仅限于耳蜗黑素细胞，这表明各种组织中的黑素细胞根据其位置相互分化。
Mammalian pigment cells produce melanin as the main pigment. Melanocytes, one of the two types of mammalian pigment cells, differentiate from the neural crest and migrate to a variety of organs during development. Melanocytes exist not only in the skin but also in other sites such as the cochlea where they are essential for hearing. Mitfmi‐bw is one of the known recessive alleles of the mouse microphthalmia‐associated transcription factor (Mitf) locus, which is essential for the development of pigment cells. Homozygous Mitfmi‐bw/Mitfmi‐bw mice have a completely white coat with black eyes and are deaf due to the lack of melanocytes. By comparing gene expression profiles in cochleae of wild‐type and Mitfmi‐bw/Mitfmi‐bw mice, we now demonstrate the specific expression of glutathione S‐transferase alpha 4 (Gsta4) in the stria vascularis. Gsta4 encodes one of the cytosolic glutathione S‐transferases (GSTs) which participate in detoxification processes of many tissues. This gene is specifically expressed in intermediate cells of the stria vascularis, suggesting a novel function for cochlear melanocytes. Moreover, among mammalian pigment cells, expression of Gsta4 was restricted to cochlear melanocytes, suggesting that melanocytes in various tissues differentiate from one another depending on their location.