Identification, fine mapping and characterization of Rht-dp, a recessive wheat dwarfing (reduced height) gene derived from Triticum polonicum
Identification, fine mapping and characterization of Rht-dp, a recessive wheat dwarfing (reduced height) gene derived from Triticum polonicum
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源自小麦的隐性小麦矮化（高度降低）基因 Rht-dp 的鉴定、精细定位和表征
DOI：
10.1007/s13258-012-0022-z
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发表时间：
2012-10-01
期刊：
影响因子：
2.1
通讯作者：
Zhou, Yong-Hong
中科院分区：
文献类型：
--
作者：
Kang, Hou-Yang;Lin, Li-Juan;Zhou, Yong-Hong
半矮化性状是一项重要的农艺学性状，在育种中具有抗风雨害（抗倒伏）和稳定高产的作用。矮秆波兰小麦（Triticum polonicum L., 2n = 4x = 28, AABB AS304）是普通小麦矮秆及其他性状改良的潜在供体。用AS304与高品种AS302杂交获得的F-2群体及其衍生的F-2:3系进行遗传分析，发现AS304携带一个隐性矮化基因，暂称为Rht-dp。利用分子标记和散装分离分析对该基因进行了表征和定位。在染色体臂4BS上定位了8个多态性SSR标记（Xwmc511、Xgwm495、xgwm113、Xgwm192、Xgpw7026、Xgpw3017、Xgpw1108和Xgpw7521）和2个AFLP标记(M (8) /E(5)和M （4) /E(3)）。距离Rht-dp最近的连锁标记Xgpw3017和M (8) /E(5)分别位于0.5 cM和3.5 cM处，将使其标记辅助转移到小麦育种群体中。等位基因检测表明，Rht-dp与Rht-B1b具有等位基因；因此，它可能是Rht-B1位点的另一个等位基因。
Semi-dwarfism is an agronomically important trait in breeding for resistance to damage by wind and rain (lodging resistance) and for stable high yields. Dwarf Polish wheat (Triticum polonicum L., 2n = 4x = 28, AABB AS304) is a potential donor of dwarfing and other traits for common wheat improvement. A genetic analysis using an F-2 population derived from a cross of AS304 and tall cultivar AS302 and derived F-2:3 lines indicated that AS304 carries a recessive dwarfing gene, temporarily designated Rht-dp. Molecular markers and bulked segregant analysis were used to characterize and map the gene. Eight polymorphic SSR markers (Xwmc511, Xgwm495, Xgwm 113, Xgwm192, Xgpw7026, Xgpw3017, Xgpw1108 and Xgpw7521) on chromosome arm 4BS and two AFLP markers (M (8) /E (5) and M (4) /E (3) ) were mapped relative to the dwarfing locus. The closest linked markers, Xgpw3017 and M (8) /E (5) at 0.5 and 3.5 cM, respectively, from Rht-dp will enable its marker assisted transfer to wheat breeding populations. Allelic tests indicated that Rht-dp was allelic to Rht-B1b; hence it may be an alternative allele at the Rht-B1 locus.