Variation in growth rate between Arabidopsis ecotypes is correlated with cell division and A-type cyclin-dependent kinase activity
Variation in growth rate between Arabidopsis ecotypes is correlated with cell division and A-type cyclin-dependent kinase activity
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DOI：
10.1104/pp.002923
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发表时间：
2002-06-01
期刊：
影响因子：
7.4
通讯作者：
Inzé, D
中科院分区：
文献类型：
--
作者：
Beemster, GTS;De Vusser, K;Inzé, D
我们使用运动学分析来研究导致拟南芥 18 个生态型之间初生根生长变化的生长过程。最慢的（Landsbergecta，71 mum h(-1)）和生长最快的品系（Wassilewskija [Ws]；338 Am h(-1)）之间的根伸长率相差4倍。这种差异几乎同样是由成熟皮质细胞长度（84 mum [Landsbergecta] 至 237 mum [Ws]）和细胞生产速率（0.63 cell h(-1) [NW108] 至 1.83 cell h(-1) [Ws]）的变化造成的。反过来，细胞产量由细胞周期持续时间的变化（19 小时 [Tsu] 至 48 小时 [NW108]）决定，并且在较小程度上由分裂细胞数量的差异（32 [Weiningen] 至 61 [Ws]）决定。我们发现成熟细胞大小和核内复制之间没有相关性，驳斥了两者相关的假设。然而，细胞生产率和细胞周期蛋白依赖性激酶（CDKA）的活性之间存在很强的相关性。该蛋白质的水平可以解释 32% 的 CDKA 变异。因此，CDKA 的调节因子，例如细胞周期蛋白和抑制剂，也可能参与其中。这些数据提供了受遗传差异影响的细胞周期调节和全植物生长速率之间的功能联系。
We used a kinematic analysis to investigate the growth processes responsible for variation in primary root growth between 18 ecotypes of Arabidopsis. Root elongation rate differed 4-fold between the slowest (Landsberg erecta, 71 mum h(-1)) and fastest growing line (Wassilewskija [Ws]; 338 Am h(-1)). This difference was contributed almost equally by variations in mature cortical cell length (84 mum [Landsberg erecta] to 237 mum [Ws]) and rate of cell production (0.63 cell h(-1) [NW108] to 1.83 cell h(-1) [Ws]). Cell production, in turn, was determined by variation in cell cycle duration (19 h [Tsu] to 48 h [NW108]) and, to a lesser extent, by differences in the number of dividing cells (32 [Weiningen] to 61 [Ws]). We found no correlation between mature cell size and endoreduplication, refuting the hypothesis that the two are linked. However, there was a strong correlation between cell production rates and the activity of the cyclin-dependent kinase (CDKA). The level of the protein could explain 32% of the variation in CDKA. Therefore, it is likely that regulators of CDKA, such as cyclins and inhibitors, are also involved. These data provide a functional link between cell cycle regulation and whole-plant growth rate as affected by genetic differences.