Effects of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment on ovarian development of the sapphire devil, Chrysiptera cyanea
Effects of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment on ovarian development of the sapphire devil, Chrysiptera cyanea
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神经毒素1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）处理对蓝魔鬼鱼卵巢发育的影响
DOI：
10.1007/s10695-014-0006-9
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发表时间：
2015
影响因子：
2.9
通讯作者：
Takemura A
中科院分区：
文献类型：
--
作者：
Badruzzaman M;Imamura S;Takeuchi Y;Ikegami T;Takemura A
在控制鱼类生殖的神经内分泌系统中，多巴胺（DA）是一种促性腺激素抑制因子，对某些物种的性腺发育具有调节作用。本研究探讨了化学破坏多巴胺能神经元在大脑中的DA生产和卵巢发育的蓝宝石devilChrysiptera cyanea，一个礁相关联的雀鲷。抗生物素蛋白-生物素-过氧化物酶复合物的方法，使用的抗体对酪氨酸羟化酶（TH），在DA合成途径中的关键酶，确定了一个人口的多巴胺能神经元与胞体在前腹视前核的间脑和纤维终止于近侧部的垂体远侧。在含有0.02和0.2 µg/mL 1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）的海水中饲养鱼类2天，导致DA、3，4-二羟基苯乙酸（DOPAC; DA代谢物）和全脑DA代谢率降低。0.02 μg/mL MPTP处理2天后，间脑TH阳性神经元数量减少。这些结果表明，MPTP治疗破坏间脑TH阳性神经元，从而减少DA的合成和释放从大脑。这种治疗可以在产卵季节挽救卵巢被人为收缩的鱼类的卵巢发育。MPTP处理后第5和第7天的性腺指数显著高于对照组。卵母细胞在卵黄发生阶段观察到MPTP处理的鱼，但在对照鱼的卵巢。这些结果表明，大脑中的DA驱动了蓝宝石魔鬼的卵巢发育。
In the neuroendocrine system controlling fish reproduction, dopamine (DA) acts as a gonadotropin inhibitory factor and plays a role in regulating gonadal development of certain species. The present study examined the effects of chemical destruction of dopaminergic neurons in the brain on DA production and ovarian development in the sapphire devilChrysiptera cyanea, a reef-associated damselfish. The avidin–biotin–peroxidase complex method using an antibody against tyrosine hydroxylase (TH), a critical enzyme in the DA synthesis pathway, identified a population of dopaminergic neurons with somata in the anteroventral preoptic nucleus of the diencephalon and fibers terminating in the proximal pars distalis of the pituitary. Maintaining fish in seawater containing 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) at 0.02 and 0.2 µg/mL for 2 days resulted in decreases in DA, 3,4-dihydroxyphenylacetic acid (DOPAC; DA metabolite), and DA metabolic rate in the whole brain. The number of TH-positive neurons in the diencephalon decreased after 0.02 µg/mL MPTP treatment for 2 days. These results suggest that MPTP treatment destroys TH-positive neurons in the diencephalon, thereby decreasing the synthesis and release of DA from the brain. This treatment rescued ovarian development in fish with artificially retracted ovaries during the spawning season. The gonadosomatic index of MPTP-treated fish 5 and 7 days after treatment was significantly higher than that of control fish. Oocytes in the vitellogenic stages were observed in the ovaries of MPTP-treated fish, but not in control fish. These results suggest that DA in the brain drives ovarian development in the sapphire devil.