Induction of type III secretion by cell-free Chlamydia trachomatis elementary bodies.
Induction of type III secretion by cell-free Chlamydia trachomatis elementary bodies.
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无细胞沙眼衣原体原体诱导 III 型分泌。
DOI：
10.1016/j.micpath.2008.10.002
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发表时间：
2008-11
影响因子：
3.8
通讯作者：
Hackstadt, Ted
中科院分区：
文献类型：
--
作者：
Jamison, Wendy P.;Hackstadt, Ted
衣原体在其发育周期的两个不同阶段分泌III型效应蛋白。初级体（EBs）分泌至少一种预形成的效应蛋白，Tarp，从细胞外的位置穿过宿主的质膜。一旦内化，一组新转录的蛋白质被分泌出来修饰包涵膜。为了更好地了解衣原体III型分泌的触发因素，并开发鉴定新效应物的方法，我们研究了其他革兰氏阴性细菌系统中T3SS的各种诱诱剂，以Tarp分泌作为激活指标，确定它们是否能够激活EBs衣原体III型分泌。暴露于FBS、BSA或鞘脂和富含胆固醇的脂质体（SCRL）可诱导衣原体EBs分泌Tarp。钙螯合剂EGTA存在时，FBS和BSA的诱导作用增强，而scl则没有。这种分泌是温度依赖性的，并被EBs的多聚甲醛固定所抑制。
Chlamydiae secrete type III effector proteins at two distinct stages of their developmental cycle. Elementary bodies (EBs) secrete at least one pre-formed effector protein, Tarp, across the host plasma membrane from an extracellular location. Once internalized, a set of newly transcribed proteins are secreted to modify the inclusion membrane. In an effort to better understand the triggers for chlamydial type III secretion and develop means to identify new effectors, we investigated various inducers of T3SS in other Gram-negative bacterial systems to determine if they were able to activate chlamydial type III secretion from EBs using Tarp secretion as an indicator of activation. Chlamydial EBs are induced to secrete Tarp by exposure to FBS, BSA, or sphingolipid and cholesterol-rich liposomes (SCRL). The induction by FBS and BSA, but not SCRL, is enhanced in the presence of the calcium-chelator, EGTA. This secretion was temperature dependent and inhibited by paraformaldehyde fixation of the EBs.
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影响因子：
3.1
作者：
Bahrani, FK;Sansonetti, PJ;Parsot, C
通讯作者：
Parsot, C
影响因子：
3.6
作者：
Fields, KA;Mead, DJ;Hackstadt, T
通讯作者：
Hackstadt, T
影响因子：
3.6
作者：
Fields, KA;Hackstadt, T
通讯作者：
Hackstadt, T
DOI：
10.1073/pnas.0603044103
发表时间：
2006-10-17
影响因子：
11.1
作者：
Jewett, Travis J.;Fischer, Elizabeth R.;Hackstadt, Ted
通讯作者：
Hackstadt, Ted
影响因子：
11.4
作者：
Niebuhr, K;Giuriato, S;Payrastre, B
通讯作者：
Payrastre, B