HIGH-TITER PACKAGING CELLS PRODUCING RECOMBINANT RETROVIRUSES RESISTANT TO HUMAN SERUM
HIGH-TITER PACKAGING CELLS PRODUCING RECOMBINANT RETROVIRUSES RESISTANT TO HUMAN SERUM
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DOI：
10.1128/jvi.69.12.7430-7436.1995
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发表时间：
1995-12-01
影响因子：
5.4
通讯作者：
COLLINS, MKL
中科院分区：
文献类型：
--
作者：
COSSET, FL;TAKEUCHI, Y;COLLINS, MKL
设计新型逆转录病毒蛋白表达构建体以保留最小逆转录病毒序列并通过在病毒基因表达后重新启动翻译来表达显性选择标记。选择HT 1080细胞作为生产细胞，因为它们能够释放对人血清灭活具有抗性的高滴度病毒。描述了两种基于HT 1080的包装细胞系，其产生具有嗜酸性鼠白血病病毒（FLYA 13系）或猫内源性病毒RD 114（FLYRD 18系）的包膜糖蛋白的莫洛尼鼠白血病病毒核心。与先前的逆转录病毒包装系统的直接比较表明，FLYA13和FLYRD18系释放的无辅助病毒重组病毒的滴度高100倍。
Novel retroviral protein expression constructs were designed to retain minimal retroviral sequences and to express dominant selectable markers by reinitiation of translation after expression of the viral genes, HT1080 cells were selected as producer cells for their ability to release high-titer viruses that are resistant to inactivation by human serum. Two HT1080-based packaging cell lines which produce Moloney murine leukemia virus cores with envelope glycoproteins of either amphotropic murine leukemia virus (FLYA13 line) or cat endogenous virus RD114 (FLYRD18 line) are described. Direct comparison with previous retroviral packaging systems indicated that 100-fold-higher titers of helper-free recombinant viruses were released by the FLYA13 and FLYRD18 lines.