The chromosomal constitution of postmitotic neurons, assessed by neuronal nuclear transfer into oocytes and in ES cell lines derived from them
The chromosomal constitution of postmitotic neurons, assessed by neuronal nuclear transfer into oocytes and in ES cell lines derived from them
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有丝分裂后神经元的染色体构成，通过神经元核转移到卵母细胞及其衍生的 ES 细胞系中进行评估
DOI：
10.1159/000230004
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发表时间：
2009
影响因子：
1.7
通讯作者：
T. Yagi
中科院分区：
文献类型：
--
作者：
T. Osada;N. Kakazu;M. Watanabe;H. Yamane;T. Yagi
采用光谱核型分析技术对神经核(N-NT)或卵丘细胞核(C-NT)移植到卵母细胞及其胚胎干细胞衍生体(NTES细胞)所产生的胚胎的染色体组成进行了分析。我们在第一次有丝分裂分裂和NT胚胎的浓缩染色单体中检测到了染色体的变化。我们还在神经核克隆的NT胚胎来源的NTES细胞中发现了克隆性易位。显示染色体重排的NTES细胞的分化潜能受到部分限制。我们的发现表明，平衡或不平衡的染色体易位可以发生在NT胚胎发生的早期，这表明DNA修复系统在NT胚胎发生和NTES细胞建立过程中都被激活。我们观察到N-NT胚胎的染色体改变发生率高于C-NT胚胎，这可能反映了神经元基因组中双链(DS)DNA断裂的更高频率。
Spectral karyotyping (SKY) was used to assess the chromosomal constitution of embryos generated by nuclear transfer (NT) of neuronal nuclei (N-NT) or cumulus cell nuclei (C-NT) into oocytes and of their embryonic stem cell derivatives (ntES cells). We detected chromosomal changes during the first mitotic cleavage and in the condensed chromatids of NT embryos. We also found clonal translocations in the ntES cells that were derived from NT embryos cloned from neuronal nuclei. The differentiation potentials of the ntES cells showing chromosomal rearrangements were partly restricted. Our findings indicate that balanced or unbalanced chromosomal translocations can occur in early NT embryogenesis, suggesting that a DNA repair system is activated during both NT embryogenesis and ntES cell establishment. We observed a higher incidence of chromosomal changes in N-NT than in C-NT embryos, which may reflect a higher frequency of double-stranded (ds) DNA breaks in the neuronal genome.