Immune toxicity of phenanthrene and its combined effects of white spot syndrome virus on the survival of kuruma shrimp (Penaeus Japonicus)
Immune toxicity of phenanthrene and its combined effects of white spot syndrome virus on the survival of kuruma shrimp (Penaeus Japonicus)
复制标题
菲的免疫毒性及其与白斑综合症病毒对日本对虾存活的联合影响
DOI：
10.1016/j.ecoenv.2020.111640
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发表时间：
2021
影响因子：
6.8
通讯作者：
Ito Katsutoshi
中科院分区：
文献类型：
--
作者：
Ito Mana;Satoh Jun;Hano Takeshi;Mekata Tohru;Ito Katsutoshi
在人类经常出没的海岸栖息的虾暴露于各种污染物。此外，在这些环境中还观察到对虾种群造成严重损害的病毒感染。本研究旨在评估菲（Phe），在沿海环境中检测到的污染物，对库鲁马虾（对虾）的免疫毒性效应。我们进一步研究了在Phe（30或300 μg/L）和白色斑点综合征病毒（WSSV）联合暴露后对虾的存活率。结果表明，暴露于苯丙氨酸7天后，免疫系统相关参数，包括血细胞总数和血淋巴中酚氧化酶活性降低（p< 0.05）。然而，在暴露三天后没有检测到这些影响。此外，联合暴露试验显示，在暴露于300 μg/L Phe和感染WSSV后，对虾死亡率增加。WSSV基因拷贝数也观察到这些共同暴露的虾增加。综上所述，这些结果表明，长期接触苯丙氨酸损害了P.导致WSSV的致命性增殖。因此，考虑到Phe和WSSV的联合暴露导致虾的死亡率增加，必须考虑和控制由多种应力引起的有害影响，在kuruma虾居住的地区。
Shrimp inhabiting coasts that are frequented by humans are exposed to various pollutants. Additionally, viral infections that cause serious damage to shrimp populations have been observed in these environments. The present study sought to evaluate the immunotoxic effects of phenanthrene (Phe), a pollutant detected in coastal environments, on kuruma shrimp (Penaeus japonicus). We further examined the survival of shrimp following combined exposure to Phe (30 or 300 μg/L) and white spot syndrome virus (WSSV). Results show that exposure to Phe for seven days decreased immune system-related parameters, including total hemocyte count and phenoloxidase activity in hemolymph (p< 0.05). However, these effects were not detected after three days of exposure. Moreover, a combined exposure assay revealed that shrimp mortality increased following exposure to 300 μg/L Phe and infection with WSSV. The number of WSSV gene copies was also observed to increase in these co-exposed shrimp. Taken together, these results indicate that long-term Phe exposure impairs the immune system ofP. japonicus, resulting in fatal proliferation of WSSV. Hence, considering that combined exposure to Phe and WSSV leads to increased mortality of shrimp, it is imperative that the detrimental effects elicited by multiple stresses be considered, and controlled, in areas inhabited by kuruma shrimp.