Cellular responses and cytokine production in post-treatment hookworm patients from an endemic area in Brazil
Cellular responses and cytokine production in post-treatment hookworm patients from an endemic area in Brazil
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DOI：
10.1111/j.1365-2249.2004.02449.x
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发表时间：
2004-05-01
影响因子：
4.6
通讯作者：
Corrêa-Oliveira, R
中科院分区：
文献类型：
--
作者：
Geiger, SM;Massara, CL;Corrêa-Oliveira, R
人类钩虫感染广泛分布在热带地区，对宿主发病率和人类健康有重大影响。在本研究中，我们调查了最近接受化疗的美洲钩虫感染的学童外周血单核细胞（PBMC）的细胞反应性和细胞因子的产生，并将其与非感染的地方病对照组进行比较。钩虫病人和治疗，鸡蛋阴性的个人表现出较低的细胞反应性对植物血凝素（PHA）和钩虫抗原相比，鸡蛋阴性的地方性控制。感染患者和接受治疗的卵子阴性个体的PBMC中促炎性肿瘤坏死因子-α（TNF-α）的基线产生升高。另一方面，PHA-或钩虫抗原诱导的白细胞介素（IL）-12和干扰素（IFN）-γ的分泌高于地方病控制比钩虫患者，谁继续鸡蛋阳性或鸡蛋阴性治疗后。此外，来自地方病对照组的PBMC比其他患者组分泌更多的IL-5和IL-13。与此相反，自发以及抗原驱动的IL-10分泌较低，地方病对照组与其他组相比。总之，明确钩虫感染以及卵阴性治疗的患者公开了促炎性TNF-α的自发细胞分泌升高，调节性Th 2型IL-10的显著分泌和IL-12、IFN-γ、IL-5和IL-13的产生受损。
Human hookworm infections are distributed widely in tropical areas and have a significant impact on host morbidity and human health. In the present study, we investigated the cellular responsiveness and cytokine production in peripheral blood mononuclear cells (PBMC) from Necator americanus-infected schoolchildren who had recently received chemotherapy, and compared them with non-infected endemic controls. Hookworm patients and treated, egg-negative individuals showed a lower cellular reactivity against phytohaemagglutinin (PHA) and hookworm antigen when compared with egg-negative endemic controls. The baseline production of proinflammatory tumour necrosis factor-alpha (TNF-alpha) in PBMC from infected patients and treated, egg-negative individuals was elevated. On the other hand, PHA- or hookworm antigen-induced interleukin (IL)-12 and interferon (IFN)-gamma secretion was higher in endemic controls than in hookworm patients, who either continued egg-positive or were egg-negative after treatment. Also, PBMC from endemic controls secreted more IL-5 and IL-13 than the other patient groups. Opposite to that, the spontaneous as well as the antigen-driven IL-10 secretion was lower in endemic controls when compared with the other groups. In summary, patently hookworm-infected as well as egg-negative treated patients disclosed an elevated spontaneous cellular secretion of proinflammatory TNF-alpha, a prominent secretion of regulatory Th2-type IL-10 and an impaired production of IL-12, IFN-gamma, IL-5 and IL-13.