Characterization and regulation of new secondary metabolites from Aspergillus ochraceus M18 obtained by UV mutagenesis
Characterization and regulation of new secondary metabolites from Aspergillus ochraceus M18 obtained by UV mutagenesis
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DOI：
10.1139/w04-117
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Lebrihi, A
中科院分区：
文献类型：
--
作者：
Awad, G;Mathieu, F;Lebrihi, A
紫外线照射赭曲霉NRRL 3174分生孢子导致赭曲霉毒素和青霉酸途径的稳定突变。这些突变体，特别是M18，产生了出乎意料的大量新代谢产物。通过TLC和HPLC分离纯化得到两个新化合物，并确定了其化学结构。它们是2，10-二甲基-4-羟基-6-氧代-4-十一烯-7-炔（1）和4-（3-甲基-2-丁烯基）氧基1-苯乙酸（2）。化合物1对革兰氏阳性细菌如金黄色葡萄球菌和枯草芽孢杆菌非常有活性，但对革兰氏阴性细菌、真菌和酵母无活性。然而，化合物2没有抗菌活性。1的产生通常与生长有关，而化合物2的产生与生长无关。这两种代谢产物的生物合成受碳源和氮源的影响。
UV irradiation of Aspergillus ochraceus NRRL 3174 conidia led to stable mutations in ochratoxin and penicillic-acid pathways. These mutants, especially M18, produced an unexpectedly large number of new metabolites. Two new compounds were purified by TLC and HPLC and their chemical structures were determined. They are 2,10-dimethyl 4-hydroxy-6-oxo-4-undecen-7-yne (1) and 4-(3-methyl-2- butenyl) oxy 1-phenyl acetic acid (2). Compound 1 is very active against Gram-positive bacteria, such as Staphylococcus aureus and Bacillus subtilis, but inactive against Gram-negative bacteria, fungi, and yeasts. However, compound 2 has no antibiotic activity. The production of 1 was generally associated with growth, whereas that of compound 2 was dissociated from growth. The biosynthesis of these 2 metabolites was influenced by the sources of carbon and nitrogen.