Characterization of pyrene degradation by Pseudomonas sp strain Jpyr-1 isolated from active sewage sludge
Characterization of pyrene degradation by Pseudomonas sp strain Jpyr-1 isolated from active sewage sludge
复制标题
从活性污水污泥中分离出的假单胞菌 Jpyr-1 菌株降解芘的表征
DOI：
10.1016/j.biortech.2013.03.184
复制
发表时间：
2013-07-01
影响因子：
11.4
通讯作者：
Jia, Lingyun
中科院分区：
文献类型：
--
作者：
Ma, Jing;Xu, Li;Jia, Lingyun
以芘为唯一碳源，从活性污泥中分离到一株新的多环芳烃（PAHs）降解菌。经16 S rRNA基因序列分析，该菌株被鉴定为假单胞菌（Pseudomonasp. Jpyr-1）。芘初始浓度为200 mg L-1时，培养48 h，最大降解速率为3.07 mg L-1h（-1）。在芘和多环芳烃的二元体系中，Jpyr-1的酶系统对芘表现出较强的选择性。此外，芘降解的竞争抑制由其他多环芳烃化合物发生在二元体系。Jpyr-1对苯并蒽、苯并[a]芘、苯并[a]芘等多环芳烃也有较强的降解能力。此外，在芘降解过程中检测到几种代谢产物，表明Jpyr-1通过邻苯二甲酸酯途径降解芘。综上所述，这些结果表明假单胞菌Jpyr-1是一种新的多环芳烃降解菌株，可能适用于多环芳烃污染场地的生物修复。(C)2013爱思唯尔有限公司版权所有。
Using pyrene as a sole carbon, a new polycyclic aromatic hydrocarbons (PAHs)-degrading bacterial strain was isolated from the active sewage sludge. This strain was identified as Pseudomonas sp. Jpyr-1 by 16S rRNA gene sequence analysis. The maximum degradation rate of pyrene was 3.07 mg L-1 h(-1) in 48 h incubation with initial pyrene concentration of 200 mg L-1. Moreover, in binary system consisting of pyrene and another PAH, the enzyme system of Jpyr-1 showed a preference toward pyrene. Furthermore, competitive inhibition of pyrene degradation by other PAH compounds occurred in the binary system. Jpyr-1 could also rapidly degrade other PAHs, such as benzanthracene, chrysene and benzo[a]pyrene. Moreover, several metabolites were detected during pyrene degradation which indicated that Jpyr-1 degraded pyrene through the o-phthalate pathway. Taken together, these results indicated that Pseudomonas sp. Jpyr-1 was a new PAHs-degrading strain that might be useful in the bioremediation of sites contaminated with PAHs. (C) 2013 Elsevier Ltd. All rights reserved.