Vacuolar localization of wound-induced carboxypeptidase inhibitor in potato leaves.
Vacuolar localization of wound-induced carboxypeptidase inhibitor in potato leaves.
复制标题
马铃薯叶片中伤口诱导的羧肽酶抑制剂的液泡定位。
DOI：
10.1104/pp.78.1.76
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发表时间：
1985
期刊：
影响因子：
7.4
通讯作者：
Clarence A. Ryan
中科院分区：
文献类型：
--
作者：
Heike Holländer;Julie K. Andersen;Clarence A. Ryan
马铃薯叶片中的创伤诱导的羧肽酶抑制剂被证明定位于中央液泡的细胞。通过免疫测定（ELISA）定量从5- 6周龄马铃薯植物的上部未受伤叶分离的原生质体和液泡中的抑制剂，所述马铃薯植物在48小时前在其下部叶上受伤以诱导羧肽酶抑制剂的积累。抑制剂的合成和区室化的调节类似于马铃薯和番茄叶片中的创伤诱导的丝氨酸蛋白酶抑制剂I和II，并且似乎是针对攻击害虫的诱导防御反应的一部分。
The wound-induced carboxypeptidase inhibitor in potato leaves was shown to be localized in the central vacuoles of the cells. The inhibitor was quantified by immunological assays (ELISA) in protoplasts and vacuoles isolated from upper unwounded leaves of 5- to 6-week old potato plants that had been wounded on their lower leaves 48 hours earlier to induce the accumulation of the carboxypeptidase inhibitor. The regulation of the synthesis and compartmentation of the inhibitor is similar to that of wound-induced serine proteinase Inhibitors I and II in potato and tomato leaves and appears to be part of an induced defense response against attacking pests.