Brief daily exposure to low-intensity vibration mitigates the degradation of the intervertebral disc in a frequency-specific manner
Brief daily exposure to low-intensity vibration mitigates the degradation of the intervertebral disc in a frequency-specific manner
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DOI：
10.1152/japplphysiol.00846.2011
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发表时间：
2011-12-01
影响因子：
3.3
通讯作者：
Judex, Stefan
中科院分区：
文献类型：
--
作者：
Holguin, Nilsson;Uzer, Gunes;Judex, Stefan
Holguin N, Uzer G, Chiang F-P, Rubin C, Judex S.每日短暂暴露于低强度振动中减轻椎间盘退化的频率特异性方式。中国生物医学工程学报，2011,31(2):444 - 444。首次发表于2011年9月29日；doi: 10.1152 / japplphysiol.00846.2011。-大鼠后肢卸车导致椎间盘快速萎缩，并降低椎间盘高度和糖胺聚糖含量。在这里，我们测试了一个假设，即低强度的机械振动（0.2 g），作为运动的替代品，将减轻这种退化。4组sd大鼠（4.5月龄，n = 11/组）后肢卸荷4周。在两个HU组中，通过将大鼠置于垂直振荡频率为45或90 Hz （HU + 45, HU + 90）的平台上，以直立姿势中断卸载15分钟/天。假对照大鼠在无活性板上站立15分钟/天（HU + SC）。将这三个实验组与不间断负重（HU）的HU和正常行走年龄匹配的对照组进行比较。在HU和HU + SC大鼠中，卸载4周导致IVD高度降低10%，整个IVD中糖胺聚糖（7%）和髓核（17%）减少，整个IVD中胶原与糖胺聚糖的比例增加（17%）。每天短暂接触90赫兹的机械振荡可以减轻这种退化；与HU +/- SC相比，HU + 90的IVD高8%，整个IVD中糖胺聚糖含量（12%）和髓核中糖胺聚糖含量（24%）更高。相比之下，45 Hz信号未能减轻脊柱负荷改变带来的身高或糖胺聚糖含量的变化，但使胶原与糖胺聚糖的比例恢复到与年龄匹配的对照组的水平。总之，卸荷引起IVD明显的表型和生化变化，这种恶化不会因短暂的负重而减慢。然而，低强度的90hz振动叠加在负重上，在很大程度上保留了IVD的形态和生物化学，这表明这些基于生物力学的信号可能有助于在长时间不活动时保护IVD。
Holguin N, Uzer G, Chiang F-P, Rubin C, Judex S. Brief daily exposure to low-intensity vibration mitigates the degradation of the intervertebral disc in a frequency-specific manner. J Appl Physiol 111: 1846-1853, 2011. First published September 29, 2011; doi:10.1152/japplphysiol.00846.2011.-Hindlimb unloading of the rat causes rapid hypotrophy of the intervertebral disc (IVD) as well as reduced IVD height and glycosaminoglycan content. Here we tested the hypothesis that low-intensity mechanical vibrations (0.2 g), as a surrogate for exercise, will mitigate this degradation. Four groups of Sprague-Dawley rats (4.5 mo, n = 11/group) were hindlimb unloaded (HU) for 4 wk. In two of the HU groups, unloading was interrupted for 15 min/day by placing rats in an upright posture on a platform that was vertically oscillating at 45 or 90 Hz (HU + 45, HU + 90). Sham control rats stood upright on an inactive plate for 15 min/day (HU + SC). These three experimental groups were compared with HU uninterrupted by weightbearing (HU) and to normally ambulating age-matched controls. In the HU and HU + SC rats, 4 wk of unloading resulted in a 10% smaller IVD height, as well as less glycosaminoglycan in the whole IVD (7%) and nucleus pulposus (17%) and a greater collagen-to-glycosaminoglycan ratio in the whole IVD (17%). Brief daily exposure to 90 Hz mechanical oscillations mitigated this degradation; compared with HU +/- SC, the IVD of HU + 90 had an 8% larger height and greater glycosaminoglycan content in the whole IVD (12%) and nucleus pulposus (24%). In contrast, the 45 Hz signal failed to mitigate changes in height or glycosaminoglycan content brought with altered spinal loading, but normalized the collagen-to-glycosaminoglycan ratio to levels observed in age-matched controls. In summary, unloading caused marked phenotypic and biochemical changes in the IVD, a deterioration that was not slowed by brief weightbearing. However, low-intensity 90 Hz vibrations superimposed on weightbearing largely preserved the morphology and biochemistry of the IVD and suggest that these biomechanically based signals may help protect the IVD during long bouts of nonambulation.