The regeneration and screening of watercress somaclones for resistance to Spongospora subterranea f. sp. nasturtii and measurement of somaclonal variation
The regeneration and screening of watercress somaclones for resistance to Spongospora subterranea f. sp. nasturtii and measurement of somaclonal variation
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DOI：
10.1023/a:1006042817075
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发表时间：
1998-01-01
影响因子：
3
通讯作者：
Blakesley, D
中科院分区：
文献类型：
--
作者：
Claxton, JR;Arnold, DL;Blakesley, D
以西洋菜（Rorippa nasturtium-aquaticum）茎、下胚轴、真叶、子叶和叶柄为外植体，在含有不同浓度噻苯隆和2，4-二氯苯氧乙酸的Murashige和Skoog培养基上培养愈伤组织，研究其再生不定芽的能力。茎愈伤组织在含5 μ M噻苯隆和0.05 μ M 2，4-二氯苯氧乙酸的培养基上形成的芽再生率最高，平均每个相应外植体18个芽。组织学研究证实，芽直接起源于愈伤组织。25%的体细胞无性系表现出叶片形状、株高、腋生分枝或倍性的体细胞无性系变异。6%的变异可遗传到自交一代。筛选协议的开发，以允许体细胞无性系的鉴定与经济上具有破坏性的豆瓣菜根病原体，海绵孢subdichea f。旱金莲虽然883体细胞无性系筛选使用该协议，没有显着增加抗病性检测。
A range of watercress (Rorippa nasturtium-aquaticum) explants (stems, hypocotyls, true-leaves, cotyledons and petioles) were tested for their capacity to regenerate adventitious shoots from callus formed using Murashige and Skoog medium containing different concentrations of thidiazuron and 2,4-dichlorophenoxyacetic acid. The highest shoot regeneration rate was a mean of 18 shoots per responding explant from stem callus formed on medium containing 5 mu M thidiazuron and 0.05 mu M 2,4-dichlorophenoxyacetic acid. A histological study confirmed that shoots originated directly from callus tissue. Twenty five percent of somaclones exhibited somaclonal variation in leaf shape, plant height, axillary branching or ploidy. The variation in 6% of somaclones was heritable to the first selfed generation. A screening protocol was developed to permit the identification of somaclones with increased resistance to the economically damaging watercress root pathogen, Spongospora subterranea f. sp. nasturtii. Although 883 somaclones were screened using this protocol, no significant increase in disease resistance was detected.