Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10
Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10
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DOI：
10.3892/or.2012.2039
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发表时间：
2012-12-01
期刊：
影响因子：
4.2
通讯作者：
Hirano, Kazuyuki
中科院分区：
文献类型：
--
作者：
Iguchi, Kazuhiro;Hayakawa, Yuri;Hirano, Kazuyuki
低细胞外pH和低营养的瘤内区域是实体瘤的常见特征。虽然癌细胞通常会在从环境中移除时死亡，但仍有一小部分细胞存活。在本研究中，分离前列腺癌细胞系LNCaP的亚系LNCaP-F10，并检查其低pH/低营养耐受特性。LNCaP-F10细胞在低pH/低营养条件下生长，这导致LNCaP细胞的细胞死亡。细胞死亡与寡核体DNA片段化和聚（ADP-核糖）聚合酶（PARP）裂解，表明低pH/低营养诱导这些细胞的凋亡。BCL 2、BIRC 5和DARK 1的表达在LNCaP-F10和LNCaP细胞中检测到显著差异。在不存在或存在前列腺基质细胞刺激的情况下，将LNICaP-F10细胞植入裸鼠肾包膜下空间所引起的肿瘤生长大于植入LNCaP细胞所引起的肿瘤生长。LNCaP-F10细胞在体外对低pH/低营养环境诱导的细胞凋亡具有抵抗性，在体内表现出恶性潜能。
Intratumoral regions of low extracellular pH and low nutrition are common features of solid tumors. Although cancer cells normally die when they are removed from their environment, a small population of cells survive. In the present study, the subline LNCaP-F10, of the prostate cancer cell line LNCaP, was isolated and its low pH/low nutrient-resistant properties were examined. LNCaP-F10 cells were grown under low-pH/low-nutrient conditions, which caused cell death of the LNCaP cells. The cell death was associated with oligonucleosomal DNA fragmentation and poly (ADP-ribose) polymerase (PARP) cleavage, indicating that low-pH/low-nutrient induced apoptosis in these cells. Significant differences in the expression of BCL2, BIRC5 and DARK1 were detected between LNCaP-F10 and LNCaP cells. Tumor growth caused by implantation of LNICaP-F10 cells into the renal subcapsular space of nude mice in the absence or presence of prostate stromal cell stimulation was greater than that caused by implantation of LNCaP cells. LNCaP-F10 cells were resistant to apoptosis induced by an environment of low-pH/low-nutrient in vitro, and displayed malignant potential in vivo.