The cancer stem cell marker CD133 has high prognostic impact but unknown functional relevance for the metastasis of human colon cancer
The cancer stem cell marker CD133 has high prognostic impact but unknown functional relevance for the metastasis of human colon cancer
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DOI：
10.1002/path.2597
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发表时间：
2009-12-01
影响因子：
7.3
通讯作者：
Jung, Andreas
中科院分区：
文献类型：
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作者：
Horst, David;Scheel, Silvio K.;Jung, Andreas
在结肠癌中，CD133最近被用于富集具有肿瘤启动能力的肿瘤细胞亚群，因此被建议用于标记结肠癌干细胞。然而，令人惊讶的是，这种分子已被证明对肿瘤发生本身缺乏功能重要性。在此，我们研究了CD133是否可能与患者结肠癌转移有关，并且由于转移除了肿瘤起始外还需要其他一些生物学特性，因此我们研究了敲低CD133表达对结肠癌细胞系增殖、迁移、侵袭和集落形成的影响。在匹配的病例对照收集中，我们证明高CD133表达与同步肝转移密切相关，而sirna介导的该因子的敲低对上述生物学特性没有显著影响。因此，我们得出结论，CD133表达是对结肠癌具有高预后影响的标志物，而它似乎没有明显的功能作用作为这种恶性肿瘤的驱动力。版权所有(C) 2009大不列颠和爱尔兰病理学会。约翰·威利父子有限公司出版。
In colon cancer, CD133 has recently been used to enrich for a subset of tumour cells with tumour-initiating capabilities and was therefore suggested to mark colon cancer stem cells. However, this molecule has surprisingly been shown to lack functional importance for tumour initiation itself. Herein, we investigated whether CD133 may be relevant for colon cancer metastasis in patients, and as metastasis requires several additional biological characteristics besides tumour initiation, we examined the effects of knocking down CD133 expression in colon cancer cell lines on proliferation, migration, invasion, and colony formation. We demonstrate that high CD133 expression correlates strongly with synchronous liver metastasis in a matched case-control collection, while siRNA-mediated knock down of this factor has no significant effect on the mentioned biological characteristics. Thus, we conclude that CD133 expression is a marker with high prognostic impact for colon cancer, while it seems to have no obvious functional role as a driving force of this malignancy. Copyright (C) 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.