STRUCTURAL STUDIES ON THE LINKAGE UNIT OF RIBITOL TEICHOIC-ACID OF LACTOBACILLUS-PLANTARUM
STRUCTURAL STUDIES ON THE LINKAGE UNIT OF RIBITOL TEICHOIC-ACID OF LACTOBACILLUS-PLANTARUM
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DOI：
10.1111/j.1432-1033.1985.tb08802.x
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
ITO, E
中科院分区：
文献类型：
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作者：
KOJIMA, N;ARAKI, Y;ITO, E
对植物乳杆菌 AHU 1413 细胞壁中核糖醇磷壁酸与肽聚糖连接的连接单元进行了结构研究。在 pH 2.5 下加热细胞壁导致仅释放 5% 的核糖醇磷壁酸成分作为水溶性物质。 N-乙酰化后对细胞壁进行相同的处理导致释放约80％的磷壁酸部分，得到磷壁酸连接的糖制剂，其含有约等摩尔量的甘露糖胺、葡糖胺和甘油作为次要成分。通过温和的碱处理将磷壁酸连接的糖水解成二糖。 N-乙酰甘露糖胺基(β1.fwdarw.4)N-乙酰葡糖胺和核糖醇磷壁酸与甘油连接。 N-乙酰化细胞壁的史密斯降解产生特征片段，1，2-乙二醇-磷酸-甘油-磷酸-N-乙酰甘露糖胺基(β1→4)N-乙酰葡糖胺。当完整的细胞壁经过 NaNO2 处理，然后进行 NaBH4 还原时，核糖醇磷壁酸部分在水溶性聚合物级分中被回收，通过弱碱处理从其中释放出糖，N-乙酰甘露糖胺基-2,5-脱水甘露醇。这些结果得出这样的结论：该生物体完整细胞壁中的核糖醇磷壁酸链通过独特的连接单元甘油-磷酸-N-乙酰甘露糖胺基(β1fwdarw4)葡糖胺与肽聚糖连接。磷壁酸部分和肽聚糖之间的连接对酸水解的异常稳定性似乎是由于连接两种聚合物的磷酸二酯桥中的 N-未取代的葡萄糖胺残基所致。
Structural studies were carried out on the linkage unit which joins ribitol teichoic acid to peptidoglycan in the cell walls of L. plantarum AHU 1413. The heating of the cell walls at pH 2.5 led to release of only 5% of ribitol teichoic acid components as water-soluble material. The same treatment of the cell walls after N-acetylation led to release of about 80% of the teichoic acid moiety, giving a teichoic-acid-linked sugar preparation which contained about equimolar amounts of mannosamine, glucosamine and glycerol as minor components. The teichoic-acid-linked sugar was hydrolyzed by mild alkaline treatment into a disaccharide. N-acetylmannosaminyl(.beta. 1 .fwdarw. 4)N-acetylglucosamine and ribitol teichoic acid linked to glycerol. The Smith degradation of the N-acetylated cell walls gave a characteristic fragment, 1,2-ethylenediol-phospho-glycerol-phospho-N-acetylmannosaminyl(.beta. 1 .fwdarw. 4)N-acetylglucosamine. When the intact cells walls were subjected to the NaNO2 treatment followed by NaBH4 reduction, the ribitol teichoic acid moiety was recovered for the most part in the water-soluble polymer fraction, from which a sugar, N-acetylmannosaminyl-2,5-anhydromannitol, was released by mild alkaline treatment. These results lead to the conclusion that the ribitol teichoic acid chain in the intact cell walls of this organism is linked to peptidoglycan through a unique linkage unit, glycerol-phospho-N-acetylmannosaminyl(.beta.1 .fwdarw. 4) glucosamine. The anomalous stability of the linkage between the teichoic acid moiety and peptidoglycan against acid hydrolysis seems to be accounted for by the involvement of the N-unsubstituted glucosamine residue in the phosphodiester bridge that joins the 2 polymers.