The Anticancer Effect of Inula viscosa Methanol Extract by miRNAs' Re-regulation: An in vitro Study on Human Malignant Melanoma Cells
The Anticancer Effect of Inula viscosa Methanol Extract by miRNAs' Re-regulation: An in vitro Study on Human Malignant Melanoma Cells
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DOI：
10.1080/01635581.2020.1869791
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发表时间：
2021-02-04
影响因子：
2.9
通讯作者：
Tunca, Berrin
中科院分区：
文献类型：
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作者：
Colak, Dilara Kamer;Egeli, Unal;Tunca, Berrin
由于化疗效果有限，恶性黑色素瘤 (MM) 是最致命的皮肤癌类型，因此需要替代和自然疗法。在本研究中，我们评估了旋覆花甲醇和水提取物（IVM 和 IVW）对 MM 细胞、A2058 和 MeWo 以及正常成纤维细胞的抗癌潜力。经过色谱和抗氧化活性分析后，确定它们在24-72小时内随剂量增加的抗增殖作用。与 IVW 相比，IVM 以剂量和时间依赖性方式在 MM 细胞中诱导更多的细胞死亡。这种效果可能是由于其中酚类物质含量较高所致。 IVM 显着诱导 MM 细胞比成纤维细胞更多的凋亡死亡 (p < 0.01)，这也在形态学上得到了支持。 IVM 还分别导致 A2058 和 MeWo 的细胞周期停滞在 G0/G1 和 G2/M 期，并抑制 MM 细胞的迁移能力 (p < 0.01)。此外，发现 IVM 在调节 MM 细胞中的 MM 相关 miRNA、上调 miR-579 和 miR-524 以及下调 miR-191 和 miR-193 方面具有显着潜力 (p < 0.05，p < 0.01)。因此，首次证明了 IVM 通过调节 miRNA 表达的抗癌作用。因此，具有这些潜力的 IVM 可能是 MM 治疗的有希望的候选者。
Alternative and natural therapies are needed for malignant melanoma (MM), the most deadly skin cancer type due to chemotherapy's limited effect. In the present study, we evaluated the anticancer potentials of Inula viscosa methanol and water extracts (IVM and IVW) on MM cells, A2058 and MeWo, and normal fibroblasts. After the chromatographic and antioxidant activity analysis, their antiproliferative effects were determined with the increasing doses for 24-72 h. IVM induced more cell death in a dose and time-dependent manner in MM cells compared to IVW. This effect was probably due to the higher amount of phenolics in it. IVM significantly induced more apoptotic death in MM cells than fibroblasts (p < 0.01), which was also supported morphologically. IVM also caused cell cycle arrest at G0/G1 and G2/M phases in A2058 and MeWo, respectively, and suppressed the migration ability of MM cells (p < 0.01). Additionally, IVM was found to have significant potential in regulating MM-related miRNAs, upregulating miR-579 and miR-524, and downregulating miR-191 and miR-193, in MM cells (p < 0.05, p < 0.01). As a result, the anticancer effect of IVM via regulating miRNAs' expression has been demonstrated for the first time. Thus, IVM, with these potentials, may be a promising candidate for MM treatment.