The Two Cryptochrome/Photolyase Family Proteins Fulfill Distinct Roles in DNA Photorepair and Regulation of Conidiation in the Gray Mold Fungus Botrytis cinerea
The Two Cryptochrome/Photolyase Family Proteins Fulfill Distinct Roles in DNA Photorepair and Regulation of Conidiation in the Gray Mold Fungus Botrytis cinerea
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DOI：
10.1128/aem.00812-17
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发表时间：
2017-09-01
影响因子：
4.4
通讯作者：
Schumacher, Julia
中科院分区：
文献类型：
--
作者：
Cohrs, Kim C.;Schumacher, Julia
灰葡萄孢（Botrytis cinerea）是一种重要的植物病原真菌，其无性分化程序受环境光照条件的严格调控。菌核在恒定的黑暗中形成;黑色/近紫外（NUV）光诱导分生孢子形成;蓝光抑制两种分化程序。黑色/NUV光的感测归因于隐花色素/光裂合酶家族（CPF）的蛋白质。为了阐明光诱导分生孢子形成的分子基础，我们对B基因组中编码的两种CPF蛋白进行了功能表征。灰葡萄作为推定的积极作用的成分。B。cinerea β 1（Bcase 1）是一种环丁烷嘧啶二聚体（CPD）光裂合酶，是光驱动的DNA修复（光复活）的主要酶，在信号传导中没有明显作用。相反，属于cry-DASH蛋白质的Bc 12蛋白被抑制光修复，但通过在白色，特别是黑色/NUV光中抑制分生孢子形成来执行调节功能。bccry 1和bccry 2的转录是由光诱导的，在一个白色衣领复合物（WCC）依赖的方式，但无论是光还是WCC是必不可少的抑制分生孢子通过Bccry 2时，bccry 2是组成型表达。此外，Bcadher 2影响WCC诱导和WCC抑制基因的转录水平，表明WCC下游的信号传导功能。由于这两种CPF蛋白质都是由黑色/NUV光诱导的，这种效应的起源仍然难以捉摸，可能与一个未知的UV光响应system.IMPORTANCE灰葡萄孢是一种经济上重要的植物病原体，导致灰霉病在各种各样的植物物种，包括高价值的作物和观赏花卉。病害在田间的传播依赖于分生孢子的形成，这一过程受不同光质的调节。虽然这一特征已经被发现很久了，但我们才刚刚开始了解其潜在的分子机制。B中的分生孢子。灰霉病由黑色/近紫外光诱导，其感测归因于隐花色素/光裂合酶家族（CPF）蛋白的作用。在这里，我们报告的不同功能的两个CPF蛋白的光响应的B。灰叶虽然BcB 1在光保护中作为主要的光裂合酶，但BcB 2作为隐花色素在调节光形态发生（抑制分生孢子形成）中具有信号功能。
The plant-pathogenic leotiomycete Botrytis cinerea is known for the strict regulation of its asexual differentiation programs by environmental light conditions. Sclerotia are formed in constant darkness; black/near-UV (NUV) light induces conidiation; and blue light represses both differentiation programs. Sensing of black/NUV light is attributed to proteins of the cryptochrome/photolyase family (CPF). To elucidate the molecular basis of the photoinduction of conidiation, we functionally characterized the two CPF proteins encoded in the genome of B. cinerea as putative positive-acting components. B. cinerea CRY1 (BcCRY1), a cyclobutane pyrimidine dimer (CPD) photolyase, acts as the major enzyme of light-driven DNA repair (photoreactivation) and has no obvious role in signaling. In contrast, BcCRY2, belonging to the cry-DASH proteins, is dispensable for photorepair but performs regulatory functions by repressing conidiation in white and especially black/NUV light. The transcription of bccry1 and bccry2 is induced by light in a White Collar complex (WCC)-dependent manner, but neither light nor the WCC is essential for the repression of conidiation through BcCRY2 when bccry2 is constitutively expressed. Further, BcCRY2 affects the transcript levels of both WCC-induced and WCC-repressed genes, suggesting a signaling function downstream of the WCC. Since both CPF proteins are dispensable for photoinduction by black/NUV light, the origin of this effect remains elusive and may be connected to a yet unknown UV-light-responsive system.IMPORTANCE Botrytis cinerea is an economically important plant pathogen that causes gray mold diseases in a wide variety of plant species, including high-value crops and ornamental flowers. The spread of disease in the field relies on the formation of conidia, a process that is regulated by different light qualities. While this feature has been known for a long time, we are just starting to understand the underlying molecular mechanisms. Conidiation in B. cinerea is induced by black/near-UV light, whose sensing is attributed to the action of cryptochrome/photolyase family (CPF) proteins. Here we report on the distinct functions of two CPF proteins in the photoresponse of B. cinerea. While BcCRY1 acts as the major photolyase in photoprotection, BcCRY2 acts as a cryptochrome with a signaling function in regulating photomorphogenesis (repression of conidiation).