The FvemiR167b-FveARF6 module increases the number of roots and leaves in woodland strawberry
The FvemiR167b-FveARF6 module increases the number of roots and leaves in woodland strawberry
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DOI：
10.1016/j.scienta.2021.110692
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发表时间：
2022-02
影响因子：
4.3
通讯作者：
Tian-Yu Li;Shao-xi Wang;Xiao-Guang Tang;Xiang Dong;He Li
中科院分区：
文献类型：
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作者：
Tian-Yu Li;Shao-xi Wang;Xiao-Guang Tang;Xiang Dong;He Li
Roots and leaves are both important organs of the plant and central to the life strategy, which are crucial for plant survival and perform a wide range of functions. However, the mechanism of miR167 and its target gene,auxin response factor6(ARF6), regulating the development of roots and leaves for strawberry, has not yet been characterized. In this study, miR167b precursor andFveARF6were isolated from the woodland strawberry ‘Ruegen’ (Fragaria vesca), and RLM-5′ RACE showed thatFveARF6transcript is cleavage target of FvemiR167b. Both FvemiR167b and its target gene expressed in different organs of strawberry, and the expression ofFveARF6could be induced by IAA. Overexpressing-FvemiR167b and RNAi-FveARF6 transgenic lines both exhibited an increase in the number of roots and leaves of strawberry. Transactivation analysis showed that FveARF6 presented transcriptional activation activity in yeast. Gene expression analysis indicated that the transcript levels of the strawberryGH3family also declined in RNAi-ARF6 transgenic plants compared with the wild-type controls. TheGH3family promoter sequences of strawberry had at least one AuxRE element. Taken together, our results reveal that FvemiR167b represses the expression ofFveARF6gene to cause the roots and leaves of strawberry to grow in number, which can likely further regulate the growth of the plant byGH3family. This will help us understand the molecular mechanism of miR167-ARF6module in woodland strawberry.
