Differential gene expression in porcine SK6 cells infected with wild-type and SAP domain-mutant foot-and-mouth disease virus.
Differential gene expression in porcine SK6 cells infected with wild-type and SAP domain-mutant foot-and-mouth disease virus.
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感染野生型和 SAP 结构域突变口蹄疫病毒的猪 SK6 细胞中的差异基因表达。
DOI：
10.1007/s12250-015-3709-x
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Zheng Haixue
中科院分区：
文献类型：
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作者：
Ni Zixin;Yang Fan;Cao Weijun;Zhang Xiangle;Jin Ye;Mao Ruoqing;Du Xiaoli;Li Weiwei;Guo Jianhong;Liu Xiangtao;Zhu Zixiang;Zheng Haixue
口蹄疫病毒(FMDV)是一种高度传染性的牲畜疾病的病原体。口蹄疫病毒LproSAP结构域的突变降低了口蹄疫病毒对猪的致病性。为了确定与猪细胞致病性降低相关的基因表达谱，我们使用下一代测序技术进行了转录组分析，并将感染含有LproFMDV的SK6细胞中差异表达的基因与SAP结构域的野生型和突变版本进行了比较。这一分析产生了1,853个基因，表现出≥2倍的表达变化，并通过对几个差异表达基因的实时定量聚合酶链式反应检测得到了验证。许多与抗病毒反应相关的差异表达基因对应于转录因子、免疫调节、细胞因子产生、炎症反应和细胞凋亡相关基因。基因表达谱的改变可能是两个口蹄疫病毒变异株之间致病性差异的原因。我们的结果为进一步研究口蹄疫病毒LPro的抗病毒途径和致病机制提供了有意义的基因。
Foot-and-mouth disease virus (FMDV) is the causative agent of a highly contagious disease in livestock. The viral proteinase Lproof FMDV is involved in pathogenicity, and mutation of the LproSAP domain reduces FMDV pathogenicity in pigs. To determine the gene expression profiles associated with decreased pathogenicity in porcine cells, we performed transcriptome analysis using next-generation sequencing technology and compared differentially expressed genes in SK6 cells infected with FMDV containing Lprowith either a wild-type or mutated version of the SAP domain. This analysis yielded 1,853 genes that exhibited a ≥ 2-fold change in expression and was validated by real-time quantitative PCR detection of several differentially expressed genes. Many of the differentially expressed genes correlated with antiviral responses corresponded to genes associated with transcription factors, immune regulation, cytokine production, inflammatory response, and apoptosis. Alterations in gene expression profiles may be responsible for the variations in pathogenicity observed between the two FMDV variants. Our results provided genes of interest for the further study of antiviral pathways and pathogenic mechanisms related to FMDV Lpro.