Is absence of pyruvate dehydrogenase complete in mitochondria a possible explanation of significant aerobic glycolysis by normal human leukocytes?
Is absence of pyruvate dehydrogenase complete in mitochondria a possible explanation of significant aerobic glycolysis by normal human leukocytes?
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DOI：
10.1016/s0014-5793(98)00273-7
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发表时间：
1998-04-03
期刊：
影响因子：
3.5
通讯作者：
Ray, S
中科院分区：
文献类型：
--
作者：
Biswas, S;Ray, M;Ray, S
用不同的呼吸底物检测了健康供体和白血病患者白细胞线粒体的耗氧量，结果表明，丙酮酸不能被正常白细胞的线粒体利用，而白血病白细胞的线粒体可以利用丙酮酸作为良好的呼吸底物。对白细胞中可能存在的丙酮酸脱氢酶复合物（PDC）的研究表明，这种酶在正常白细胞的线粒体中明显不存在，但在白血病白细胞的线粒体中却相当活跃。正常白细胞线粒体中PDC的缺失可以解释在正常白细胞中观察到的显著的有氧糖酵解现象。(C) 1998年欧洲生化学会联合会。
The oxygen consumption of leukocyte mitochondria of both healthy donors and leukemic patients was tested by using different respiratory substrates, The results indicate that pyruvate could not be utilized by mitochondria of normal leukocytes, whereas mitochondria of leukemic leukocytes could use pyruvate as a good respiratory substrate, A search for the possible presence of pyruvate dehydrogenase complex (PDC) in leukocytes indicates that this enzyme is apparently absent in mitochondria of normal leukocytes but is quite active in mitochondria of leukemic leukocytes. The absence of PDC in normal leukocyte mitochondria can explain the phenomenon of significant aerobic glycolysis that has been observed in normal leukocytes. (C) 1998 Federation of European Biochemical Societies.