Pollen transmission of asparagus virus 2 (AV-2) may facilitate mixed infection by two AV-2 isolates in asparagus plants.
Pollen transmission of asparagus virus 2 (AV-2) may facilitate mixed infection by two AV-2 isolates in asparagus plants.
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DOI：
10.1094/phyto-12-13-0348-r
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发表时间：
2014-08
期刊：
影响因子：
3.2
通讯作者：
Ryusuke Kawamura;H. Shimura;T. Mochizuki;S. Ohki;C. Masuta
中科院分区：
文献类型：
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作者：
Ryusuke Kawamura;H. Shimura;T. Mochizuki;S. Ohki;C. Masuta
芦笋病毒 2 型 (AV-2) 是伊拉病毒属的成员，被认为会诱发芦笋衰退综合症。已知 AV-2 通过种子传播，25 年前就提出了花粉传播的可能性，但尚未得到验证。在 AV-2 序列分析中，我们意外地发现两种不同的 AV-2 分离株在两株芦笋植物中混合感染。由于两种相关病毒的混合感染通常通过交叉保护来防止，因此我们怀疑AV-2的花粉传播与混合感染有关。使用 AV-2 感染的烟草植物进行的免疫组织化学分析和原位杂交表明，AV-2 位于分生组织中并与花粉粒相关。为了通过花粉传播实验产生混合感染，将分别感染两种 AV-2 分离株的两株本塞姆氏烟草植物进行杂交。衍生的切割扩增多态性序列分析鉴定了子代幼苗中的每种 AV-2 分离株，表明花粉传播确实可能导致混合感染，至少在本塞姆氏烟草中是如此。
Asparagus virus 2 (AV-2) is a member of the genus Ilarvirus and thought to induce the asparagus decline syndrome. AV-2 is known to be transmitted by seed, and the possibility of pollen transmission was proposed 25 years ago but not verified. In AV-2 sequence analyses, we have unexpectedly found mixed infection by two distinct AV-2 isolates in two asparagus plants. Because mixed infections by two related viruses are normally prevented by cross protection, we suspected that pollen transmission of AV-2 is involved in mixed infection. Immunohistochemical analyses and in situ hybridization using AV-2-infected tobacco plants revealed that AV-2 was localized in the meristem and associated with pollen grains. To experimentally produce a mixed infection via pollen transmission, two Nicotiana benthamiana plants that were infected with each of two AV-2 isolates were crossed. Derived cleaved-amplified polymorphic sequence analysis identified each AV-2 isolate in the progeny seedlings, suggesting that pollen transmission could indeed result in a mixed infection, at least in N. benthamiana.