The Histone Acetyltransferase CLOCK Is an Essential Component of the Herpes Simplex Virus 1 Transcriptome That Includes TFIID, ICP4, ICP27, and ICP22
The Histone Acetyltransferase CLOCK Is an Essential Component of the Herpes Simplex Virus 1 Transcriptome That Includes TFIID, ICP4, ICP27, and ICP22
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DOI：
10.1128/jvi.00876-11
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发表时间：
2011-09-01
影响因子：
5.4
通讯作者：
Roizman, Bernard
中科院分区：
文献类型：
--
作者：
Kalamvoki, Maria;Roizman, Bernard
其他地方发表的研究表明，单纯疱疹病毒调节蛋白ICP0与组蛋白乙酰转移酶生物钟的伴侣和调节者BMAL1相互作用，这两种蛋白都定位在ND10小体上，并由病毒蛋白稳定，具有酶活性的时钟部分补充Delta ICP0突变体，而沉默时钟抑制病毒基因的表达。在这里，我们报告时钟是转录复合体的一个组成部分，包括TFIID、ICP4、ICP27和ICP22。这些结果表明，时钟组蛋白乙酰转移酶是病毒复制周期中病毒转录机制的组成部分，ICP27和ICP22作为病毒转录组的组成部分参与了病毒基因的表达。
Studies published elsewhere have shown that the herpes simplex virus regulatory protein ICP0 interacts with BMAL1, a partner and regulator of circadian histone acetyltransferase CLOCK, that both proteins localize at ND10 bodies and are stabilized by viral proteins, that enzymatically active CLOCK partially complements Delta ICP0 mutants, and that silencing of CLOCK suppresses the expression of viral genes. Here we report that CLOCK is a component of the transcriptional complex that includes TFIID, ICP4, ICP27, and ICP22. The results suggest that the CLOCK histone acetyltransferase is a component of the viral transcriptional machinery throughout the replicative cycle of the virus and that ICP27 and ICP22 initiate their involvement in viral gene expression as components of viral transcriptome.