Simultaneous measurement of contractile force and field potential of dynamically beating human iPS cell-derived cardiac cell sheet-tissue with flexible electronics
Simultaneous measurement of contractile force and field potential of dynamically beating human iPS cell-derived cardiac cell sheet-tissue with flexible electronics
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使用柔性电子设备同时测量动态跳动的人 iPS 细胞来源的心脏细胞片组织的收缩力和场电位
DOI：
10.1039/d1lc00411e
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发表时间：
2021
期刊：
影响因子：
6.1
通讯作者：
and Shinjiro Umezu
中科院分区：
文献类型：
--
作者：
Takashi Ohya;Haruki Ohtomo;Tetsutaro Kikuchi;Daisuke Sasaki;Yohei Kawamura;Katsuhisa Matsuura;Tatsuya Shimizu;Kenjiro Fukuda;Takao Someya;and Shinjiro Umezu
人诱导多能干细胞（iPS）来源的心肌细胞在世界范围内用于体外药理学和病理学研究。特别是，从iPS细胞衍生的心肌细胞产生的心脏组织的功能评估预计将提供药物作用的精确预测，从而简化药物开发过程。然而，目前在刚性基底上对心肌细胞进行电生理和收缩评估的方法不适用于动态跳动的心脏组织。在这里，我们展示了一种新的同时测量系统的收缩力和胞外场电位的iPS细胞衍生的心脏细胞片组织使用500 nm厚的柔性电子片。经证实，所开发的系统适用于药理学研究和兴奋-收缩耦合相关参数的评估，如机电窗口。我们的研究结果表明，具有心脏组织工程的柔性电子器件为药物开发提供了先进的平台。该系统的建立将为心功能的药理学研究提供新的思路。
Human induced pluripotent stem (iPS) cell-derived cardiomyocytes are used for in vitro pharmacological and pathological studies worldwide. In particular, the functional assessment of cardiac tissues created from iPS cell-derived cardiomyocytes is expected to provide precise prediction of drug effects and thus streamline the process of drug development. However, the current format of electrophysiological and contractile assessment of cardiomyocytes on a rigid substrate is not appropriate for cardiac tissues that beat dynamically. Here, we show a novel simultaneous measurement system for contractile force and extracellular field potential of iPS cell-derived cardiac cell sheet-tissues using 500 nm-thick flexible electronic sheets. It was confirmed that the developed system is applicable for pharmacological studies and assessments of excitation–contraction coupling-related parameters, such as the electro-mechanical window. Our results indicate that flexible electronics with cardiac tissue engineering provide an advanced platform for drug development. This system will contribute to gaining new insight in pharmacological study of human cardiac function.