The Absence of Thioredoxin m1 and Thioredoxin C in Anabaena sp. PCC 7120 Leads to Oxidative Stress
The Absence of Thioredoxin m1 and Thioredoxin C in Anabaena sp. PCC 7120 Leads to Oxidative Stress
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DOI：
10.1093/pcp/pcy163
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发表时间：
2018-12-01
影响因子：
4.9
通讯作者：
Hisabori, Toru
中科院分区：
文献类型：
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作者：
Deschoenmaeker, Frederic;Mihara, Shoko;Hisabori, Toru
硫氧还蛋白 (Trx) 家族蛋白在细胞中执行氧化还原调节，并且参与多种其他生物过程（例如氧化应激耐受）。在丝状蓝藻鱼腥藻属中。 PCC7120 (A. 7120) 是通过基因组分析鉴定出的八种 Trx 同工型。在这些 Trx 亚型中，Trx-m1 和 TrxC 的缺失似乎会导致 A.7120 中的氧化应激以及类囊体膜结构和藻胆蛋白组成的改变。为了彻底分析这些 Trx 干扰物的生理变化，应用了定量蛋白质组学。当然，突变体在蛋白质组中表现出类似的改变，包括藻胆蛋白的相对丰度降低以及涉及氨基酸和碳水化合物代谢的蛋白质水平增加。然而，结果还表明，突变体中参与活性氧解毒的不同蛋白质组的相对丰度发生了变化，例如 Delta trx-m1 中的 Fe-SOD 和 Delta trxC 中的 PrxQ，这表明 Trx-m1 和 TrxC 具有不同的功能。
Thioredoxin (Trx) family proteins perform redox regulation in cells, and they are involved in several other biological processes (e.g. oxidative stress tolerance). In the filamentous cyanobacterium Anabaena sp. PCC7120 (A. 7120), eight Trx isoforms have been identified via genomic analysis. Among these Trx isoforms, the absence of Trx-m1 and TrxC appears to result in oxidative stress in A. 7120 together with alterations of the thylakoid membrane structure and phycobiliprotein composition. To analyze the physiological changes in these Trx disruptants thoroughly, quantitative proteomics was applied. Certainly, the mutants exhibited similar alterations in the proteome including decreased relative abundance of phycobiliproteins and an increased level of proteins involved in amino acid and carbohydrate metabolism. Nevertheless, the results also indicated that the mutants exhibited changes in the relative abundance of different sets of proteins participating in reactive oxygen species detoxification, such as Fe-SOD in Delta trx-m1 and PrxQ in Delta trxC, suggesting distinct functions of Trx-m1 and TrxC.