Nucleotide sequence of the gene for the b subunit of human factor XIII.
Nucleotide sequence of the gene for the b subunit of human factor XIII.
复制标题
人因子 XIII b 亚基基因的核苷酸序列。
DOI：
10.1021/bi00503a007
复制
发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Davie,EW
中科院分区：
文献类型：
--
作者：
Bottenus,RE;Ichinose,A;Davie,EW
Ralph E. Bottenus、Akitada Ichinose和Earl W. Davie** Department of Biochemistry，University of华盛顿，西雅图，华盛顿98195 1990年5月22日接收; 1990年8月10日接收修订版摘要：因子XIII（Mr 320000）是一种稳定和加强纤维蛋白凝块的凝血因子。它以四聚体形式在血液中循环，由两个a亚基（每个Mr 75 000）和两个B亚基（每个Mr 80 000）组成。B亚基由641个氨基酸组成，包括10个60个氨基酸的串联重复序列，称为GP-I结构、短共有重复序列（SCR）或sushi结构域。在本研究中，人基因的B亚基已被分离，从三个不同的基因组文库中制备的噬菌体。15个独立的噬菌体插入编码整个基因的分离和限制性酶切图谱，Southernblotting，和DNA测序的特点。该基因长28个碱基，由12个外显子（I-XII）组成，中间有11个插入序列。前导序列由外显子I编码，而蛋白质的羰基末端区域由外显子XII编码。外显子II-XI分别编码一个sushi结构域，表明该基因是通过外显子改组和重复进化的。该基因的12个外显子大小为64 - 222个碱基对，内含子大小为87 - 9970个核苷酸，占基因的92%。内含子含有四个Alu重复序列，内含子A、E、I和J各一个。第五个Alu重复序列存在于基因的侧翼3 '端。在内含子中还发现两个部分Kpnl重复，包括一个在内含子I中，一个在内含子J中。内含子J中的Kpnl重复与编码人β珠蛋白的基因侧翼的约2200个核苷酸的序列和来自存在于人因子VIII的基因中的LI插入的约3800个核苷酸的序列具有89%的同源性。内含子H还包含一个“O”家族重复序列，而两个潜在的区域内的内含子G和J的Z-DNA被确定。一个核苷酸的变化被发现在该基因的编码区时，其序列进行比较的cDNA。然而，这种差异并没有导致蛋白质氨基酸序列的变化。
Ralph E. Bottenus, Akitada Ichinose, and Earl W. Davie** Department of Biochemistry, University of Washington, Seattle, Washington 98195 Received May 22, 1990; Revised Manuscript Received August 10, 1990 abstract: Factor XIII (Mr 320000) is a blood coagulation factorthat stabilizes and strengthens the fibrin clot. It circulates in blood as a tetramer composed of two a subunits (Mr 75 000 each) and two b subunits (Mr 80000 each). The b subunit consists of 641 amino acids and includes 10 tandem repeats of 60 amino acids known as GP-I structures, short consensus repeats (SCR), or sushi domains. In the present study, the human gene for the b subunit has been isolated from three different genomic libraries prepared in phage. Fifteen independent phage with inserts coding for the entire gene were isolated and characterized by restriction mapping, Southernblotting, and DNA sequencing. The gene was found to be 28 kilobases in length and consisted of 12 exons (I—XII) separated by 11 intervening sequences. The leader sequence was encoded by exon I, while the carbonyl-terminal region of the protein was encoded by exon XII. Exons II—XI each coded for a single sushi domain, suggesting that the gene evolved through exon shuffling and duplication. The 12 exons in the gene rangedin size from 64 to 222 base pairs, while the introns ranged in size from 87 to 9970 nucleotides and made up 92% of the gene. The introns contained four Alu repetitive sequences, one each in introns A, E, I, and J. A fifth Alu repeat was present in the flanking 3'end of the gene. Two partial Kpnl repeats were also found in the introns, including one in intron I and one in intron J. The Kpnl repeat in intron J was 89% homologous to a sequence of approximately 2200 nucleotides flanking the gene coding for human ß globin and approximately 3800 nucleotides from the LI insertion present in the gene for human factor VIII. Intron H also contained an “O” family repeat, while two potential regions for Z-DNA were identified within introns G and J. One nucleotide change was found in the coding region of the gene when its sequence was compared to that of the cDNA. This difference, however, did not result in a change in the amino acid sequence of the protein.