CHITOSAN-MEDIATED STIMULATION OF MACROPHAGE FUNCTION
CHITOSAN-MEDIATED STIMULATION OF MACROPHAGE FUNCTION
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DOI：
10.1016/0142-9612(94)90272-0
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发表时间：
1994-12-01
期刊：
影响因子：
14
通讯作者：
BALSAMO, G
中科院分区：
文献类型：
--
作者：
PELUSO, G;PETILLO, O;BALSAMO, G
根据生物相容性的现代定义，生物相容性材料不必是惰性的，而是具有生物活性。良性反应性意味着反应性必须适合预期用途。壳聚糖是一种非乙酰化或部分脱乙酰化的几丁质（β(1-4) 连接的 N-乙酰氨基葡萄糖的线性均聚物），因其明显令人满意的生物相容性而被提议作为生物材料。目前的研究表明，壳聚糖对巨噬细胞一氧化氮 (NO) 产生和趋化性具有体外刺激作用。巨噬细胞NO分泌归因于壳聚糖分子的N-乙酰葡糖胺单元而不是葡糖胺残基（分别为28和15μM NO）。此外，壳聚糖的免疫刺激作用非常特异，因为其他糖胺聚糖，如 N-乙酰基-D-甘露糖胺和 N-乙酰基-D-半乳糖胺，对 NO 产生没有影响（分别为 5 和 8）。体内实验强化了这一假设。透射电子显微镜分析发现植入后 14 天后样本中存在许多白细胞，显示出愈合过程较差（即成纤维细胞增殖和胶原沉积），这是我们动物模型此时组织修复的特征。
According to the modern definition of biocompatibility, a biocompatible material need not be inert but be bioactive. A benign reactivity implies that the reactivity has to be appropriate for the intended use. Chitosan, a non-acetylated or partially deacetylated chitin (a linear homopolymer of beta(1-4)-linked N-acetylglucosamine) has been proposed as a biomaterial because of its apparent satisfactory biocompatibility, The present investigation demonstrates that chitosan has an in vitro stimulatory effect on both macrophage nitric oxide (NO) production and chemotaxis. The macrophage NO secretion is attributed to the N-acetylglucosamine unit of the chitosan molecule rather than to the glucosamine residue (28 and 15 mu M NO respectively). Moreover, the immune stimulatory effect of chitosan was very specific since other glycosaminoglycans, such as N-acetyl-D-mannosamine and N-acetyl-D-galactosamine, had no effects on NO production (5 and 8 respectively). In vivo experiments strengthen this hypothesis. Transmission electron microscopy analysis identifies the presence of many leucocytes in the specimens after 14 d post-implantation, showing poor healing processes (i.e, fibroblast proliferation and collagen deposition) that characterize the tissue repair at this time in our animal model.