Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats
Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats
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DOI：
10.1152/ajpendo.1997.273.1.e207
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发表时间：
1997-07-01
影响因子：
5.1
通讯作者：
Drewes, LR
中科院分区：
文献类型：
--
作者：
Gerhart, DZ;Enerson, BE;Drewes, LR
一种多克隆亲和纯化的抗体的羧基末端的大鼠单羧酸转运蛋白1（MCT 1）在鸡和成年和哺乳大鼠的脑组织切片的免疫细胞化学研究中产生。该抗体在心脏、盲肠、肾脏和骨骼肌的免疫印迹和染色组织切片上鉴定出48 kDa条带，与该转运蛋白的报告分子量和细胞表达一致。在成人脑组织切片中，抗体标记了脑微血管内皮细胞、室管膜细胞、胶质限制膜和神经胶质细胞。在脑切片从3日龄至14日龄的大鼠，微血管更强烈的标记和神经胶质细胞标记较弱，从成年人的切片相比。免疫电子显微镜显示，标记存在于管腔和近管腔内皮细胞质膜上。这些结果表明，MCT 1可能发挥了重要作用，在通过乳酸和其他单羧酸盐跨越血脑屏障和哺乳大鼠可能特别依赖于这种转运体供应能量底物的大脑。
A polyclonal affinity-purified antibody to the carboxyl-terminal end of the rat monocarboxylate transporter 1 (MCT1) was generated in chickens and used in immunocytochemical studies of brain tissue sections from adult and suckling rats. The antibody identified a 48-kDa band on immunoblots and stained tissue sections of heart, cecum, kidney, and skeletal muscle, consistent with the reported molecular mass and cellular expression for this transporter. In tissue sections from adult brains, the antibody labeled brain microvessel endothelial cells, ependymocytes, glial-limiting membranes, and neuropil. In brain sections from 3- to 14-day-old rats, microvessels were much more strongly labeled and neuropil was weakly labeled compared with sections from adults. Immunoelectron microscopy indicated that labeling was present on both luminal and abluminal endothelial cell plasma membranes. These results suggest that MCT1 may play an important role in the passage of lactate and other monocarboxylates across the blood-brain barrier and that suckling rats may be especially dependent on this transporter to supply energy substrates to the brain.