Determinants of the exclusion zone in dopaminergic amacrine cell mosaics
Determinants of the exclusion zone in dopaminergic amacrine cell mosaics
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DOI：
10.1002/cne.10693
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发表时间：
2003-06-16
影响因子：
2.5
通讯作者：
Reese, BE
中科院分区：
文献类型：
--
作者：
Raven, MA;Eglen, SJ;Reese, BE
视网膜的一个基本组织特征是神经元的规则间隔分布，但我们对这种模式在发育过程中是如何出现的知之甚少。在这些视网膜马赛克中，多巴胺能无长突细胞的空间组织是独特的：使用最近邻和Vornoi域分析，我们发现多巴胺能无长突细胞既不是随机分布的，也没有达到其他视网膜细胞类型记录的规律性。自相关分析显示，存在一个禁区周围的个别多巴胺能无长突细胞和建模研究证实了这种组织，马赛克可以模拟一个最小的距离间隔规则定义的一组广泛的参数。实验研究确定了切向分散，命运的决定，和细胞死亡在建立这个禁区的相对贡献。克隆边界分析和邻近驱动运动的模拟折扣切向离散，而bcl-2过表达小鼠的数据排除了反馈抑制命运决定性的帐户。相比之下，细胞死亡似乎消除了邻近的多巴胺能无长突细胞，从而建立了单个细胞周围的禁区，反过来又创造了它们的镶嵌规律。(C)2003 Wiley-Liss，Inc.
A fundamental organizing feature of the retina is the presence of regularly spaced distributions of neurons, yet we have little knowledge of how this patterning emerges during development. Among these retinal mosaics, the spatial organization of the dopaminergic amacrine cells is unique: using nearest-neighbor and Vornoi domain analysis, we found that the dopaminergic amacrine cells were neither randomly distributed, nor did they achieve the regularity documented for other retinal cell types. Autocorrelation analysis revealed the presence of an exclusion zone surrounding individual dopaminergic amacrine cells and modeling studies confirmed this organization, as the mosaic could be simulated by a minimal distance spacing rule defined by a broad set of parameters. Experimental studies determined the relative contributions of tangential dispersion, fate determination, and cell death in the establishment of this exclusion zone. Clonal boundary analysis and simulations of proximity-driven movement discount tangential dispersion, while data from bcl-2 overexpressing mice rule out feedback-inhibitory fate-deterministic accounts. Cell death, by contrast, appears to eliminate dopaminergic amacrine cells that are within close proximity, thereby establishing the exclusion zone surrounding individual cells and in turn creating their mosaic regularity. (C) 2003 Wiley-Liss, Inc.