Distribution of G-protein-coupled receptor kinase (GRK) isoforms 2, 3, 5 and 6 mRNA in the rat brain
Distribution of G-protein-coupled receptor kinase (GRK) isoforms 2, 3, 5 and 6 mRNA in the rat brain
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DOI：
10.1016/s0006-8993(01)03046-3
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发表时间：
2001-11-01
期刊：
影响因子：
--
通讯作者：
Höllt, V
中科院分区：
文献类型：
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作者：
Erdtmann-Vourliotis, M;Mayer, P;Höllt, V
关于不同的G蛋白偶联受体激酶(GRKs)在大鼠脑内的分布，尤其是最近克隆的GRK5和GRK6亚型，人们知之甚少。本文将对四种G蛋白偶联受体激酶GRK2(βARK1)、GRK3(βARK2)、GRK5和GRK6在大鼠脑内的表达模式进行综述。我们和最近的研究表明，GRK2和GRK3在大鼠脑内广泛分布，其表达模式几乎相同。但总体而言，GRK3在大多数大脑区域的表达似乎弱于GRK2。例外的是卡列哈岛、黑质致密部分和蓝斑。GRK3mRNA在纹状体以及部分下丘脑和丘脑核团中表达很低或不表达。GRK6的表达模式也与GRK2相似。在尾壳核中，GRK6产生的杂交信号最强。GRK5采取了特殊的立场。这种形式的信息在大脑中并不普遍表达，但主要定位于边缘脑区，其中外侧隔区表达非常明显。因此，GRK5可能与奖赏和成瘾有关。因此，与对照组相比，可卡因致敏大鼠外侧隔区GRK5mRNA的表达水平更高。(C)2001年，爱思唯尔科学公司出版。
There is limited knowledge about the distribution of the different G-protein-coupled receptor kinases (GRKs) in the rat brain, especially for the recently cloned isoforms GRK5 and GRK6. In this work an overview will be given of the mRNA expression patterns of four G-protein-coupled receptor kinases, GRK2 (beta ARK1), GRK3 (beta ARK2), GRK5 and GRK6 in the rat brain. As now shown by us and recently by others GRK2 and GRK3 are widely distributed in rat brain with nearly the same expression pattern. But GRK3, in general, appeared to be weaker expressed than GRK2 in most brain areas. Exceptions were the islands of Calleja, the compact part of the substantia nigra and the locus coeruleus. GRK3 mRNA was very low expressed or absent in the striatum and in some hypothalamic and thalamic nuclei. The expression pattern of GRK6 was also similar to GRK2. In the caudate putamen GRK6 yielded the strongest hybridization signal of all GRK types. GRK5 took a special position. The message for this form was not expressed ubiquitously in the brain but was mainly localized in limbic brain regions with a very prominent expression in the lateral septal area. GRK5 may therefore be involved in reward and addiction. Accordingly, a higher expression level of GRK5 mRNA was found in the lateral septum of cocaine-sensitized rats as compared to controls. (C) 2001 Published by Elsevier Science B.V.