Differential expression of capillary VEGF isoforms following traumatic brain injury
Differential expression of capillary VEGF isoforms following traumatic brain injury
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DOI：
10.1179/016164107x204729
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发表时间：
2007-06-01
影响因子：
1.9
通讯作者：
Rafols, Jose A.
中科院分区：
文献类型：
--
作者：
Dore-Duffy, Paula;Wang, Xueqain;Rafols, Jose A.
目的：虽然已知创伤后发生血管生成，但我们试图表征创伤性脑损伤（TBI）动物毛细血管内血管内皮生长因子（VEGF）亚型、血管内皮生长因子受体2（VEGFR2）和血管生成素的表达。此外，我们试图在孤立的capillars.Methods周细胞死亡的特点：我们使用Marmarou的加速影响模型，诱导头部创伤和测量血管内皮生长因子，血管内皮生长因子受体2和血管生成素水平在孤立的毛细血管。结果：VEGF反应仅限于VEGF 120亚型。未观察到VEGF 164和VEGF 188的转录物增加。VEGFR2轻微增加，血管生成素增加。周细胞的一个子集是TUNEL阳性。讨论：这些结果显示了不同的表达模式的血管生成因子损伤后，并建议周细胞参与适应性血管生成可能会改变TBI后。
Objectives: While it is known that angiogenesis occurs after trauma, we sought to characterize the expression of vascular endothelial growth factor (VEGF) subtypes, vascular endothelial growth factor receptor 2 (VEGFR2) and angiopoietin within capillaries of animals subjected to traumatic brain injury (TBI). Further, we sought to characterize pericyte cell death in isolated capillaries.Methods: We used Marmarou's acceleration impact model to induce head trauma and measured VEGF, VEGFR2 and angiopoietin levels in isolated capillaries. TUNEL was used to determine pericyte cell death.Results: The VEGF response was restricted to the VEGF120 isoform. No increase in transcripts for VEGF164 and VEGF188 was observed. VEGFR2 was marginally increased and angiopoietin was increased. A subset of pericytes were TUNEL-positive.Discussion: These results show a distinct expression pattern of angiogenic factors following injury and suggest that pericyte involvement in adaptive angiogenesis may be altered following TBI.