Binding of monoclonal antibody A2B5 to gangliosides.
Binding of monoclonal antibody A2B5 to gangliosides.
复制标题
单克隆抗体 A2B5 与神经节苷脂的结合。
DOI：
10.1016/s0006-291x(83)80218-6
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发表时间：
1983
影响因子：
3.1
通讯作者：
Marcus,DM
中科院分区：
文献类型：
--
作者：
Kundu,SK;Pleatman,MA;Redwine,WA;Boyd,AE;Marcus,DM
单抗A2B5(Eisenbarth等人，Proc.纳特。阿卡德。SCI。(1979，76：4913-4917)，据报道能与多种神经元、胸腺上皮细胞和多肽分泌细胞发生反应，并能与脑内四唾液酸神经节苷脂发生特异性反应。我们发现，A2B5与神经节苷脂GQ1b、GD3、GD2、二唾液酸内酯四糖神经酰胺结合，并可能与GT1a结合，在薄层平板上检测抗体与神经节苷脂的结合。补体结合实验还表明，该抗体与神经节苷脂GQ1反应最强，与二唾液酸神经节苷脂GD3、GD2和二唾液酸内酯四糖神经酰胺反应最强，与GD1带GT1b反应弱，与GM3和GD1a反应很弱。这些数据表明，A2B5不能作为识别四唾液酸神经节苷脂的特异性试剂。
Monoclonal antibody A2B5 (Eisenbarth et al, Proc. Nat. Acad. Sci. (1979, 76:4913–4917), which reacts with neurons, thymic epithelium and peptide-hormone secreting cells of several species, was reported to react specifically with brain tetrasialogangliosides. We have found that A2B5 binds to gangliosides GQ1b, GD3, GD2, disialolactoneotetraosylceramide, and probably to GT1a, when assayed by an immunostaining procedure that detects binding of antibody to gangliosides on a thin-layer plate. Additional data obtained by complement fixation revealed that this antibody reacted most strongly with ganglioside GQ1balmost as well with disialogangliosides GD3, GD2and disialolactoneotetraosylceramide, weakly with GD1band GT1b, and very weakly with GM3and GD1a. These data indicate that A2B5 cannot be regarded as a specific reagent for the recognition of tetrasialogangliosides.