Proteomics identification of differentially expressed proteins associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen
Proteomics identification of differentially expressed proteins associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen
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DOI：
10.1074/mcp.m600146-mcp200
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发表时间：
2007-02-01
影响因子：
7
通讯作者：
Wang, Tai
中科院分区：
文献类型：
--
作者：
Dai, Shaojun;Chen, Taotao;Wang, Tai
大多数植物的成熟花粉在代谢上是静止的；然而，授粉后，它迅速萌发，并形成花粉管，将精子输送到胚囊中。由于目前还没有从拟南芥和水稻的模式植物中收集大量体外萌发的花粉进行转录组学和蛋白质组学研究的方法，因此缺乏关于种子萌发发育过程的分子信息。在这里，我们描述了一种获得大量体外萌发的水稻花粉的方法，用于蛋白质组学研究。对2300个蛋白质点进行双向电泳分析，发现186个蛋白质点在成熟花粉和萌发花粉中有差异表达。大多数都显示出不同的表达水平，只有66个似乎是发育阶段特有的。此外，在质谱学上鉴定了160个差异表达的蛋白质点，以匹配120个不同的蛋白质种类。这些蛋白质涉及不同的细胞和代谢过程，功能明显偏向壁代谢、蛋白质合成和降解、细胞骨架动力学和碳水化合物/能量代谢。与水稻孢子体蛋白质组相比，WALL代谢相关蛋白在差异表达蛋白和花粉蛋白质组中具有显著的特征。我们的研究还揭示了蛋白质的多种亚型和不同亚型之间的差异表达模式。这些结果为花粉功能专门化提供了新的见解。
Mature pollen from most plant species is metabolically quiescent; however, after pollination, it germinates quickly and gives rise to a pollen tube to transport sperms into the embryo sac. Because methods for collecting a large amount of in vitro germinated pollen grains for transcriptomics and proteomics studies from model plants of Arabidopsis and rice are not available, molecular information about the germination developmental process is lacking. Here we describe a method for obtaining a large quantity of in vitro germinating rice pollen for proteomics study. Two-dimensional electrophoresis of similar to 2300 protein spots revealed 186 that were differentially expressed in mature and germinated pollen. Most showed a changed level of expression, and only 66 appeared to be specific to developmental stages. Furthermore 160 differentially expressed protein spots were identified on mass spectrometry to match 120 diverse protein species. These proteins involve different cellular and metabolic processes with obvious functional skew toward wall metabolism, protein synthesis and degradation, cytoskeleton dynamics, and carbohydrate/energy metabolism. Wall metabolism-related proteins are prominently featured in the differentially expressed proteins and the pollen proteome as compared with rice sporophytic proteomes. Our study also revealed multiple isoforms and differential expression patterns between isoforms of a protein. These results provide novel insights into pollen function specialization.