Histone acetylation of 45S rDNA correlates with disrupted nucleolar organization during heat stress response in Zea mays L.
Histone acetylation of 45S rDNA correlates with disrupted nucleolar organization during heat stress response in Zea mays L.
复制标题
玉米 45S rDNA 的组蛋白乙酰化与热应激反应期间核仁组织的破坏相关。
DOI：
10.1111/ppl.13438
复制
发表时间：
2021-05-04
影响因子：
6.4
通讯作者：
Li，Lijia
中科院分区：
文献类型：
--
作者：
Yue，Mengxia;Gautam，Mayank;Li，Lijia
核仁在植物对热胁迫反应中的作用仍不清楚。我们目前的努力集中在探索潜在的机制，其中核仁解体调节热应激玉米线。本研究以玉米热敏感品系ZD 958和耐热品系ZDH为材料，研究了热胁迫对玉米核仁破坏的影响。免疫荧光染色显示，随着处理时间的延长，核仁破坏程度增加。热处理后，在ZD 958系中观察到核仁组织的显著变化，但ZDH系显示出轻微的改变。在正常条件下，放线菌素D（ActD）诱导的核仁分裂可抑制玉米的生长。热处理后，ZD 958的45 S rDNA H3 K9 ac和H4 K5 ac的水平显著升高，同时5 '-外转录间隔区（ETS）的转录水平也显著升高;而ZDH的组蛋白乙酰化水平和该位点的转录起始水平略有升高。据我们所知，这是第一份报告提供了一个比较洞察热应激，rDNA组蛋白修饰和核仁解体之间的耐热ZDH相比，热敏线ZD 958。我们的研究可能有助于玉米育种家通过利用表观遗传学靶向核仁获得耐热品系。本文受版权保护。All rights reserved.
The role of the nucleolus in plant response to heat stress remains largely obscure. Our current efforts focused on exploring the underlying mechanism by which nucleolar disorganization is regulated in heat stressed-maize lines. Here, two maize lines, a heat-sensitive line, ZD958 and a heat-tolerant line, ZDH, were submitted to heat stress for investigating their association with the nucleolar disruption. Immunofluorescence staining showed that nucleolar disruption increased with prolonged treatment time. After heat treatment, a significant change in nucleolus organization was observed in the ZD958 line, but the ZDH line showed mild alteration. Moreover, Actinomycin D (ActD)-induced nucleolus fission led to inhibition of maize growth under the normal condition. The ZD958 line exhibited a significant increase in the level of H3K9ac and H4K5ac of the 45S rDNA accompanied by a higher transcription of the 5'-external transcribed spacer (ETS) region, while the line ZDH showed a slight increase in histone acetylation levels and the transcriptional initiation at this site after heat treatment. To our knowledge, this is the first report providing a comparative insight between heat stress, rDNA histone modifications and nucleolus disintegration in a heat tolerant ZDH compared with a heat sensitive line ZD958. Our investigation might assist maize breeders in obtaining heat-tolerant lines by targeting nucleoli using epigenetics. This article is protected by copyright. All rights reserved.